Identification of putative candidate genes for water stress tolerance in canola (Brassica napus)

Zhang, Jing, Mason, Annliese S., Wu, Jian, Liu, Sheng, Zhang, Xuechen, Luo, Tao, Redden, Robert, Batley, Jacqueline, Hu, Liyong and Yan, Guijun (2015) Identification of putative candidate genes for water stress tolerance in canola (Brassica napus). Frontiers in Plant Science, 6 NOVEMBER: . doi:10.3389/fpls.2015.01058

Author Zhang, Jing
Mason, Annliese S.
Wu, Jian
Liu, Sheng
Zhang, Xuechen
Luo, Tao
Redden, Robert
Batley, Jacqueline
Hu, Liyong
Yan, Guijun
Title Identification of putative candidate genes for water stress tolerance in canola (Brassica napus)
Journal name Frontiers in Plant Science   Check publisher's open access policy
ISSN 1664-462X
Publication date 2015-11-27
Year available 2015
Sub-type Article (original research)
DOI 10.3389/fpls.2015.01058
Open Access Status DOI
Volume 6
Total pages 13
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Collection year 2016
Language eng
Formatted abstract
Drought stress can directly inhibit seedling establishment in canola (Brassica napus), resulting in lower plant densities and reduced yields. To dissect this complex trait, 140 B. napus accessions were phenotyped under normal (0.0 MPa, S0) and water-stressed conditions simulated by polyethylene glycol (PEG) 6000 (−0.5 MPa, S5) in a hydroponic system. Phenotypic variation and heritability indicated that the root to shoot length ratio was a reliable indicator for water stress tolerance. Thereafter, 66 accessions (16 water stress tolerant, 34 moderate and 16 sensitive lines) were genotyped using 25,495 Brassica single nucleotide polymorphisms (SNPs). Genome-wide association studies (GWAS) identified 16 loci significantly associated with water stress response. Two B. napus accessions were used for RNA sequencing, with differentially-expressed genes under normal and water-stressed conditions examined. By combining differentially-expressed genes detected by RNA sequencing with significantly associated loci from GWAS, 79 candidate genes were identified, of which eight were putatively associated with drought tolerance based on gene ontology of Arabidopsis. Functional validation of these genes may confirm key drought-related genes for selection and breeding in B. napus. Our results provide insight into the genetic basis of water stress tolerance in canola.
Keyword Canola
Water stress tolerance
Genome-wide association study
RNA sequencing
Drought-related genes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Centre for Integrative Legume Research Publications
School of Agriculture and Food Sciences
Official 2016 Collection
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Citation counts: TR Web of Science Citation Count  Cited 2 times in Thomson Reuters Web of Science Article | Citations
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