Detection of emergent strains of West Nile virus with a blood screening assay

Faddy, Helen M., Flower, Robert L.P., Seed, Clive R., Ismay, Susan, Ong, Edgar, Linnen, Jeffrey M., Cory, Robin, Holmberg, Jerry A., Hall, Roy A., Setoh, Yin X., Deerain, Joshua M. and Prow, Natalie A. (2015) Detection of emergent strains of West Nile virus with a blood screening assay. Transfusion, 56 6: 1503-1507. doi:10.1111/trf.13443


Author Faddy, Helen M.
Flower, Robert L.P.
Seed, Clive R.
Ismay, Susan
Ong, Edgar
Linnen, Jeffrey M.
Cory, Robin
Holmberg, Jerry A.
Hall, Roy A.
Setoh, Yin X.
Deerain, Joshua M.
Prow, Natalie A.
Title Detection of emergent strains of West Nile virus with a blood screening assay
Journal name Transfusion   Check publisher's open access policy
ISSN 1537-2995
0041-1132
Publication date 2015-12-08
Year available 2015
Sub-type Article (original research)
DOI 10.1111/trf.13443
Open Access Status Not Open Access
Volume 56
Issue 6
Start page 1503
End page 1507
Total pages 5
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Collection year 2016
Language eng
Formatted abstract
Background: West Nile virus (WNV) is a threat to transfusion safety. WNV Kunjin strain (WNVKUN) is endemic across parts of Australia; however, human infection is believed to be infrequent and is often associated with relatively minor symptoms. A virulent strain, closely related to WNVKUN (termed WNVNSW2011) was recently identified as the etiologic agent of encephalitis in Australian horses. The aim of this project was to investigate whether a commercially available WNV blood screening assay can detect different strains of WNVKUN, including the virulent WNVNSW2011, in human blood donor samples.

Study Design and Methods: 
Plasma samples were spiked with four different strains of WNVKUN, as well as a prototype WNV strain, at high, medium, and low viral loads. Spiking was confirmed with real-time reverse transcription–polymerase chain reaction (RT-PCR), before testing with the Procleix WNV transcription-mediated amplification (TMA) blood screening assay (Grifols).

Results: All WNV strains used were detectable by RT-PCR after being spiked into plasma. Additionally, all viral spiked samples were reactive by WNV TMA.

Conclusion: 
We experimentally demonstrate that a commercially available WNV blood screening assay can detect different strains of WNVKUN. Given that WNV can be transfusion transmissible, it is essential to confirm that emergent strains are detectable by existing blood screening methods.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
School of Medicine Publications
 
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Created: Fri, 11 Dec 2015, 10:04:14 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences