Airway dysbiosis: Haemophilus influenzae and Tropheryma in poorly controlled asthma

Simpson, Jodie L., Daly, Joshua, Baines, Katherine J., Yang, Ian A., Upham, John W., Reynolds, Paul N., Hodge, Sandra, James, Alan L., Hugenholtz, Philip, Willner, Dana and Gibson, Peter G. (2016) Airway dysbiosis: Haemophilus influenzae and Tropheryma in poorly controlled asthma. European Respiratory Journal, 47 3: 792-800. doi:10.1183/13993003.00405-2015

Author Simpson, Jodie L.
Daly, Joshua
Baines, Katherine J.
Yang, Ian A.
Upham, John W.
Reynolds, Paul N.
Hodge, Sandra
James, Alan L.
Hugenholtz, Philip
Willner, Dana
Gibson, Peter G.
Title Airway dysbiosis: Haemophilus influenzae and Tropheryma in poorly controlled asthma
Journal name European Respiratory Journal   Check publisher's open access policy
ISSN 1399-3003
Publication date 2016-03-01
Year available 2015
Sub-type Article (original research)
DOI 10.1183/13993003.00405-2015
Open Access Status Not yet assessed
Volume 47
Issue 3
Start page 792
End page 800
Total pages 9
Place of publication Lausanne, Switzerland
Publisher European Respiratory Society
Collection year 2017
Language eng
Formatted abstract
Asthma is a chronic inflammatory disorder of the airways where bacteria may act as protagonists of chronic inflammation. Little is known about the relation of airway inflammation to the presence of specific bacterial taxa. We sought to describe the sputum microbiome in adults with poorly controlled asthma.

DNA was extracted from induced sputum and microbial communities were profiled using 16S rRNA pyrosequencing. Bacterial species were characterised, and the relationship between microbial populations, asthma inflammatory subtypes and other covariates was explored. Real-time PCR was used to identify Tropheryma whipplei and Haemophilus influenzae in sputum.

Adults with neutrophilic asthma had reduced bacterial diversity and species richness. Tropheryma was identified and confirmed with real-time PCR in 12 (40%) participants. Haemophilus occurred most often in a group of younger atopic males with an increased proportion of neutrophils. PCR confirmed the presence of H. influenzae in 35 (76%) participants with poorly controlled asthma.

There are phenotype-specific alterations to the airway microbiome in asthma. Reduced bacterial diversity combined with a high prevalence of H. influenzae was observed in neutrophilic asthma, whereas eosinophilic asthma had abundant T. whipplei.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
School of Medicine Publications
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 11 Dec 2015, 09:57:01 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences