Transcriptome and proteome of Conus planorbis identify the nicotinic receptors as primary target for the defensive venom

Jin, Ai-Hua, Vetter, Irina, Himaya, Siddhihalu W. A., Alewood, Paul F., Lewis, Richard J. and Dutertre, Sébastien (2015) Transcriptome and proteome of Conus planorbis identify the nicotinic receptors as primary target for the defensive venom. Proteomics, 15 23-24: 4030-4040. doi:10.1002/pmic.201500220


Author Jin, Ai-Hua
Vetter, Irina
Himaya, Siddhihalu W. A.
Alewood, Paul F.
Lewis, Richard J.
Dutertre, Sébastien
Title Transcriptome and proteome of Conus planorbis identify the nicotinic receptors as primary target for the defensive venom
Journal name Proteomics   Check publisher's open access policy
ISSN 1615-9861
1615-9853
Publication date 2015
Sub-type Article (original research)
DOI 10.1002/pmic.201500220
Open Access Status Not Open Access
Volume 15
Issue 23-24
Start page 4030
End page 4040
Total pages 11
Place of publication Weinheim, Germany
Publisher Wiley - V C H Verlag GmbH & Co. KGaA
Collection year 2016
Language eng
Formatted abstract
Most venomous predators have evolved complex venom primarily to immobilize their prey and secondarily to defend against predators. In a new paradigm, carnivorous marine gastropods of the genus Conus were shown to rapidly and reversibly switch between two types of venoms in response to predatory or defensive stimulus, suggesting that the defensive use of venom may have a more important role in venom evolution and specialization than previously thought. To further investigate this phenomenon, the defensive repertoire of a vermivorous species, Conus planorbis, was deciphered using second-generation sequencing coupled to high-throughput proteomics. The venom gland transcriptome of C. planorbis revealed 182 unique conotoxin precursors from 25 gene superfamilies, with superfamily T dominating in terms of read and paralog numbers. Analysis of the defense-evoked venom revealed that this vermivorous species uses a similarly complex arsenal to deter aggressors as more recently evolved fish- and mollusk-hunting species, with MS/MS validating 23 conotoxin sequences from six superfamilies. Pharmacological characterization of the defensive venom on human receptors identified the nicotinic acetylcholine receptors as a primary target. This work provides the first insights into the composition and biological activity of specifically evolved defensive venoms in vermivorous cone snails.
Keyword Animal proteomics
Conotoxin
Conus planorbis
Mass spectrometry
Nicotinic receptors
Venomics
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Institute for Molecular Bioscience - Publications
 
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