Stallion sperm quality after combined ejaculate fractionation and colloidal centrifugation

Crespo, Francisco, Gosálvez, Jaime, Johnston, Stephen D. and de la Torre, Joaquina (2015) Stallion sperm quality after combined ejaculate fractionation and colloidal centrifugation. Spanish Journal of Agricultural Research, 13 4: e04SC02.1-e04SC02.6. doi:10.5424/sjar/2015134-8108


Author Crespo, Francisco
Gosálvez, Jaime
Johnston, Stephen D.
de la Torre, Joaquina
Title Stallion sperm quality after combined ejaculate fractionation and colloidal centrifugation
Journal name Spanish Journal of Agricultural Research   Check publisher's open access policy
ISSN 1695-971X
Publication date 2015-11-10
Sub-type Letter to editor, brief commentary or brief communication
DOI 10.5424/sjar/2015134-8108
Open Access Status DOI
Volume 13
Issue 4
Start page e04SC02.1
End page e04SC02.6
Total pages 6
Place of publication Madrid, Spain
Publisher Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (I N I A)
Collection year 2016
Language eng
Formatted abstract
This study investigated the possible additive benefit of ejaculate fractionation and colloidal centrifugation on stallion sperm quality. Using an open-end artificial vagina, the sperm-rich fraction (FRAC-1) was separated from the rest of the ejaculate (FRAC-2) and a third sperm sample representing the combined ejaculate was reconstituted post-ejaculation (RAW). Each semen sample was processed for colloidal centrifugation. The percentage of abnormal spermatozoa was 17.8 ± 7.0% in RAW and 14.6 ± 9.5% in FRAC-1 but decreased to 11.4 ± 4.7% and 9.6 ± 6.9% respectively, after colloidal centrifugation. A sperm DNA fragmentation index of 10.9 ± 5.1% was observed in RAW and 7.5 ± 2.4% in FRAC-1 semen collected with the AV but this decreased to 7.8 ± 2.8% and 5.2 ± 2.3% after colloidal centrifugation. The rate of increase in sperm DNA fragmentation during the first 6 h of incubation at 37 ºC was 1.8 ± 0.9% per hour in RAW semen and 2.0 ± 2.0% per hour in FRAC-1 but this significantly decreased to 1.3 ± 1.4% and 0.9 ± 0.8% respectively after colloidal centrifugation. While stallion seminal characteristics can be improved using colloidal centrifugation, further enhancement is possible if the ejaculate is initially fractionated.
Keyword Equus caballus
Ejaculate fractionation
Colloidal centrifugation
Sperm DNA fragmentation
DNA longevity
SCD
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Letter to editor, brief commentary or brief communication
Collections: School of Agriculture and Food Sciences
Official 2016 Collection
Official Audit
 
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Created: Sat, 05 Dec 2015, 20:09:05 EST by Associate Professor Stephen Johnston on behalf of School of Agriculture and Food Sciences