Profiling of free fatty acids using stable isotope tagging uncovers a role for saturated fatty acids in neuroexocytosis

Narayana, Vinod K., Tomatis, Vanesa M., Wang, Tong, Kvaskoff, David and Meunier, Frederic A. (2015) Profiling of free fatty acids using stable isotope tagging uncovers a role for saturated fatty acids in neuroexocytosis. Chemistry and Biology, 22 11: 1552-1561. doi:10.1016/j.chembiol.2015.09.010


Author Narayana, Vinod K.
Tomatis, Vanesa M.
Wang, Tong
Kvaskoff, David
Meunier, Frederic A.
Title Profiling of free fatty acids using stable isotope tagging uncovers a role for saturated fatty acids in neuroexocytosis
Journal name Chemistry and Biology   Check publisher's open access policy
ISSN 1074-5521
1879-1301
Publication date 2015-11-19
Sub-type Article (original research)
DOI 10.1016/j.chembiol.2015.09.010
Open Access Status Not Open Access
Volume 22
Issue 11
Start page 1552
End page 1561
Total pages 10
Place of publication Cambridge, MA, United States
Publisher Cell Press
Collection year 2016
Language eng
Abstract The phospholipase-catalyzed release of free fatty acids (FFAs) from phospholipids is implicated in many critical biological processes such as neurotransmission, inflammation, and cancer. However, determining the individual change in FFAs generated during these processes has remained challenging due to the limitations of current methods, and has hampered our understanding of these key mediators. Here, we developed an “iTRAQ”-like method for profiling FFAs by stable isotope tagging (FFAST), based on the differential labeling of the carboxyl group and designed to resolve analytical variance, through a multiplexed assay in cells and subcellular fractions. With nanomolar sensitivity, this method revealed a spectrum of saturated FFAs elicited during stimulation of exocytosis that was identical in neurons and neurosecretory cells. Purified secretory vesicles also generated these FFAs when challenged with cytosol. Our multiplex method will be invaluable to assess the range of FFAs generated in other physiological and pathological settings.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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