Mammalian farnesyltransferase α subunit regulates vacuolar protein sorting-associated protein 4A (Vps4A) – dependent intracellular trafficking through recycling endosomes

Marta Kubala, Norwood, Suzanne, Gomez, Guillermo, Jones, Alun, Johnston, Wayne A., Yap, Alpha S.K., Mureev, Sergey and Alexandrov, Kirill (2015) Mammalian farnesyltransferase α subunit regulates vacuolar protein sorting-associated protein 4A (Vps4A) – dependent intracellular trafficking through recycling endosomes. Biochemical and Biophysical Research Communications, 468 4: 580-586. doi:10.1016/j.bbrc.2015.10.148

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Author Marta Kubala
Norwood, Suzanne
Gomez, Guillermo
Jones, Alun
Johnston, Wayne A.
Yap, Alpha S.K.
Mureev, Sergey
Alexandrov, Kirill
Title Mammalian farnesyltransferase α subunit regulates vacuolar protein sorting-associated protein 4A (Vps4A) – dependent intracellular trafficking through recycling endosomes
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
1090-2104
Publication date 2015-11-10
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2015.10.148
Open Access Status File (Author Post-print)
Volume 468
Issue 4
Start page 580
End page 586
Total pages 22
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2016
Language eng
Formatted abstract
The protein farnesyltransferase (FTase) mediates posttranslational modification of proteins with isoprenoid lipids. FTase is a heterodimer and although the β subunit harbors the active site, it requires the α subunit for its activity. Here we explore the other functions of the FTase α subunit in addition to its established role in protein prenylation. We found that in the absence of the β subunit, the α subunit of FTase forms a stable autonomous dimeric structure in solution. We identify interactors of FTase α using mass spectrometry, followed by rapid in vitro analysis using the Leishmania tarentolae cell – free system. Vps4A was validated for direct binding to the FTase α subunit both in vitro and in vivo. Analysis of the interaction with Vps4A in Hek 293 cells demonstrated that FTase α controls trafficking of transferrin receptor upstream of this protein. These results point to the existence of previously undetected biological functions of the FTase α subunit that includes control of intracellular membrane trafficking.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 03 Dec 2015, 08:38:14 EST by Susan Allen on behalf of Institute for Molecular Bioscience