Impact of γ-chain cytokines on EBV-specific T cell cultures

Merlo, Anna, Turrini, Riccardo, Trento, Cristina, Zanovello, Paola, Dolcetti, Riccardo and Rosato, Antonio (2010) Impact of γ-chain cytokines on EBV-specific T cell cultures. Journal of Translational Medicine, 8 . doi:10.1186/1479-5876-8-121


Author Merlo, Anna
Turrini, Riccardo
Trento, Cristina
Zanovello, Paola
Dolcetti, Riccardo
Rosato, Antonio
Title Impact of γ-chain cytokines on EBV-specific T cell cultures
Journal name Journal of Translational Medicine   Check publisher's open access policy
ISSN 1479-5876
Publication date 2010-11-22
Sub-type Article (original research)
DOI 10.1186/1479-5876-8-121
Open Access Status DOI
Volume 8
Total pages 8
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: Recent preclinical adoptive immunotherapy studies in murine models prompt to employ "proper" rather than "as many as possible" antigen-specific T cells to gain better therapeutic results. Ideally, "proper" T cells are poorly differentiated in vitro, but retain the capacity to fully differentiate into effector cells in vivo, where they can undergo long-term survival and strong proliferation. Such requirements can be achieved by modifying culture conditions, namely using less "differentiating" cytokines than IL-2.

Methods: To evaluate this issue in human T cell cultures, we exploited a well characterized and clinical-grade protocol finalized at generating EBV-specific CTL for adoptive immunotherapy. In particular, we studied the impact of IL-7, IL-15 and IL-21 compared to IL-2 on different aspects of T cell functionality, namely growth kinetics, differentiation/activation marker expression, cytokine production, and short-term and long-term cytotoxicity.

Results: Results disclosed that the culture modifications we introduced in the standard protocol did not improve activity nor induce substantial changes in differentiation marker expression of EBV-specific CTL.

Conclusions: Our data indicated that the addition of γ-chain cytokines other than IL-2 for the generation of EBV-specific T cell cultures did not produce the improvements expected on the basis of recent published literature. This fact was likely due to the intrinsic differences between murine and human models and highlights the need to design ad hoc protocols rather than simply modify the cytokines added in culture.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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