Chlamydia infection and lymphomas: association beyond ocular adnexal lymphomas highlighted by multiple detection methods

Ponzoni, Maurilio, Ferreri, Andres J. M., Guidoboni, Massimo, Lettini, Antonia A., Cangi, Maria Giulia, Pasini, Elisa, Sacchi, Luciano, Pecciarini, Lorenza, Grassi, Stefano, Dal Cin, Elena, Stefano, Rosalba, Magnino, Simone, Dolcetti, Riccardo and Doglioni, Claudio (2008) Chlamydia infection and lymphomas: association beyond ocular adnexal lymphomas highlighted by multiple detection methods. Clinical Cancer Research, 14 18: 5794-5800. doi:10.1158/1078-0432.CCR-08-0676


Author Ponzoni, Maurilio
Ferreri, Andres J. M.
Guidoboni, Massimo
Lettini, Antonia A.
Cangi, Maria Giulia
Pasini, Elisa
Sacchi, Luciano
Pecciarini, Lorenza
Grassi, Stefano
Dal Cin, Elena
Stefano, Rosalba
Magnino, Simone
Dolcetti, Riccardo
Doglioni, Claudio
Title Chlamydia infection and lymphomas: association beyond ocular adnexal lymphomas highlighted by multiple detection methods
Journal name Clinical Cancer Research   Check publisher's open access policy
ISSN 1078-0432
1557-3265
Publication date 2008-09-01
Sub-type Article (original research)
DOI 10.1158/1078-0432.CCR-08-0676
Open Access Status Not yet assessed
Volume 14
Issue 18
Start page 5794
End page 5800
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher American Association for Cancer Research
Language eng
Formatted abstract
Purpose: Chlamydia psittaci (Cp) has been associated to ocular adnexal lymphomas (OAL) with variable geographic distribution. Herein, we used multiple Chlamydia detection tools to identify Cp elementary bodies - containing cell and to assess Cp prevalence in both nodal and extranodal lymphomas.

Experimental Design: TETR-PCR, immunohistochemistry, Immunofluorescence, electron microscopy, and laser-capture microdissection were done in 35 OALs to define their effect in Chlamydia detection and, moreover, to identify the Cp cellular carrier. Cp prevalence was screened by TETR-PCR in 205 extraorbital lymphomas and 135 nonneoplastic controls.

Results: Twenty-six (74%) OALs were associated with Cp infection: immunohistochemistry, immunofluorescence, and laser-capture microdissection-assisted PCR showed that monocytes/macrophages were the Cp carriers; electron microscopy showed the presence of intact Cp elementary bodies into these cells. Immunohistochemistry and TETR-PCR showed a 70% concordance rate (P - 0.001). Cp DNA was equally prevalent in non-OAL, nodal, and extranodal lymphomas: among the latter, it was more common in diffuse large B-cell lymphomas of the skin (P = 0.03) and Waldeyer's ring.

Conclusions: This multiparametric approach shows, for the first time, that monocytes/macrophages are the carriers of Cp, Cp seems preferentially associated with lymphomas arising in organs primarily exposed to antigens. The clinical implications of these findings deserve to be prospectively investigated.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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