Characterization of Epstein-Barr virus genotype in AIDS-related non-Hodgkin's lymphoma

Fassone, Lucia, Cingolani, Antonella, Martini, Maurizio, Migliaretti, Giuseppe, Oreste, Pier Luigi, Capello, Daniela, Gloghini, Annunziata, Vivenza, Daniela, Dolcetti, Riccardo, Carbone, Antonino, Antinori, Andrea, Gaidano, Gianluca and Larocca, Luigi Maria (2002) Characterization of Epstein-Barr virus genotype in AIDS-related non-Hodgkin's lymphoma. AIDS Research and Human Retroviruses, 18 1: 19-26. doi:10.1089/088922202753394682


Author Fassone, Lucia
Cingolani, Antonella
Martini, Maurizio
Migliaretti, Giuseppe
Oreste, Pier Luigi
Capello, Daniela
Gloghini, Annunziata
Vivenza, Daniela
Dolcetti, Riccardo
Carbone, Antonino
Antinori, Andrea
Gaidano, Gianluca
Larocca, Luigi Maria
Title Characterization of Epstein-Barr virus genotype in AIDS-related non-Hodgkin's lymphoma
Journal name AIDS Research and Human Retroviruses   Check publisher's open access policy
ISSN 0889-2229
1931-8405
Publication date 2002
Sub-type Article (original research)
DOI 10.1089/088922202753394682
Open Access Status Not yet assessed
Volume 18
Issue 1
Start page 19
End page 26
Total pages 8
Place of publication New Rochelle, NY, United States
Publisher Mary Ann Liebert
Language eng
Formatted abstract
In the present study sequence variations at the C terminus of the Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1), EBV-encoded latent membrane protein 1 (LMP-1), and EBNA-2 and EBNA-3C genes were investigated in 64 cases of EBV-positive AIDS-related diffuse large cell lymphoma (AIDS-DLCL), both systemic (12) and localized primarily to the central nervous system (52), and in 12 cases of EBV-positive AIDS-related Burkitt's lymphoma (AIDS-BL). Sequence analysis of the EBNA-1 C-terminal region led to the distinction of two major unrelated EBV strains, termed strain P (prototype) and strain V (variant), and their related subtypes, namely P-ala, P-thr, V-leu, V-val, and V-pro. Analysis of the LMP-1 gene was performed to assess the frequency of the C-terminus deletion variant, whereas analysis of EBNA-2 and EBNA-3C genes led to the identification of the distribution of the EBV type 1 and type 2 strains. The frequency of EBNA-1 subtypes was assessed in 49 cases of AIDS-NHL, including 37 cases of AIDS-DLCL and 12 cases of AIDS-BL. The P strain was detected in 45 of 49 cases (91.8%) whereas the V strain was found in 4 of 49 samples (8.1%). A significant difference in the distribution of the P and V strains was found between AIDS-DLCL and AIDS-BL (p < 0.01), because of the exclusive infection by the P strain of the AIDS-DLCL samples analyzed. The frequency of LMP-1 deletion variants and of EBV type 1 and type 2 strains in AIDS-DLCL overlapped with that of the general population, and no correlation was found with the evaluated clinicoepidemiological data of patients, that is, disease site, tumor histology, CD4+ cell counts, and HIV transmission route. In conclusion, we found that the distribution of the EBV genotype in all of the AIDS-NHL samples analyzed is similar to the viral representation found in control individuals of both immunocompetent and immunocompromised populations.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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