Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders

Buonaguro, Luigi, Petrizzo, Annacarmen, Tornesello, Marialina, Napolitano, Maria, Martorelli, Debora, Castello, Giuseppe, Beneduce, Gerardo, De Renzo, Amalia, Perrella, Oreste, Romagnoli, Luca, Sousa, Vitor, De Re, Valli, Dolcetti, Riccardo and Buonaguro, Franco M. (2010) Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders. Journal of Translational Medicine, 8 . doi:10.1186/1479-5876-8-18


Author Buonaguro, Luigi
Petrizzo, Annacarmen
Tornesello, Marialina
Napolitano, Maria
Martorelli, Debora
Castello, Giuseppe
Beneduce, Gerardo
De Renzo, Amalia
Perrella, Oreste
Romagnoli, Luca
Sousa, Vitor
De Re, Valli
Dolcetti, Riccardo
Buonaguro, Franco M.
Title Immune signatures in human PBMCs of idiotypic vaccine for HCV-related lymphoproliferative disorders
Journal name Journal of Translational Medicine   Check publisher's open access policy
ISSN 1479-5876
Publication date 2010-02-19
Sub-type Article (original research)
DOI 10.1186/1479-5876-8-18
Open Access Status DOI
Volume 8
Total pages 11
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Hepatitis C virus (HCV) is one of the major risk factors for chronic hepatitis, which may progress to cirrhosis and hepatocellular carcinoma, as well as for type II mixed cryoglobulinemia (MC), which may further evolve into an overt B-cell non-Hodgkin's lymphoma (NHL). It has been previously shown that B-cell receptor (BCR) repertoire, expressed by clonal B-cells involved in type II MC as well as in HCV-associated NHL, is constrained to a limited number of variable heavy (VH)- and light (VL)-chain genes. Among these, the VK3-20 light chain idiotype has been selected as a possible target for passive as well as active immunization strategy. In the present study, we describe the results of a multiparametric analysis of the innate and early adaptive immune response after ex vivo stimulation of human immune cells with the VK3-20 protein. This objective has been pursued by implementing high-throughput technologies such as multiparameter flow cytometry and multiplex analysis of cytokines and chemokines.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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