Characterization of antibodies directed against the immunoglobulin light κ chain variable chain region (VK) of hepatitis C virus-related type-II mixed cryoglobulinemia and B-cell proliferations

de Re, Valli, Simula, Maria Paola, Pavan, Alessandro, Garziera, Marica, Marin, Dolores, Dolcetti, Riccardo, de Vita, Salvatore, Sansonno, Domenico, Geremia, Silvano and Toffoli, Giuseppe (2009). Characterization of antibodies directed against the immunoglobulin light κ chain variable chain region (VK) of hepatitis C virus-related type-II mixed cryoglobulinemia and B-cell proliferations. In: 6th International Congress on Autoimmunity, Oporto Portugal, (152-160). Sep 10-14, 2008. doi:10.1111/j.1749-6632.2009.04677.x


Author de Re, Valli
Simula, Maria Paola
Pavan, Alessandro
Garziera, Marica
Marin, Dolores
Dolcetti, Riccardo
de Vita, Salvatore
Sansonno, Domenico
Geremia, Silvano
Toffoli, Giuseppe
Title of paper Characterization of antibodies directed against the immunoglobulin light κ chain variable chain region (VK) of hepatitis C virus-related type-II mixed cryoglobulinemia and B-cell proliferations
Conference name 6th International Congress on Autoimmunity
Conference location Oporto Portugal
Conference dates Sep 10-14, 2008
Journal name Contemporary Challenges in Autoimmunity   Check publisher's open access policy
Publication Year 2009
Sub-type Fully published paper
DOI 10.1111/j.1749-6632.2009.04677.x
ISBN 9781573317627
ISSN 0077-8923
Volume 1173
Start page 152
End page 160
Total pages 9
Language eng
Abstract/Summary Autoimmune type-II cryoglobulinemia (II-MC) is sustained by hepatitis C virus (HCV) infection and B-cell (oligo)clones. This is the reason why the disease may be considered an "indolent B-cell lymphoma (NHL)." B clones show a restricted use of immunoglobulin variable genes (BCR), in particular in the use of the variable κ (VK)3-2015 light chain, and show a homology between their BCR functional regions and those of autoimmune rheumatoid factors. We underlined the BCR unique repertoire with frequent rheumatoid factor activity also observed in other autoimmune disorders associated with NHL. The immunoglobulin idiotype is a clonal B-cell marker and an ideal target for immunotherapy. Five monoclonal antibodies were produced in our laboratory toward the VK3-20 of a subject with HCV infection and a II-MC-associated NHL. Epitope determination was performed using the epitope excision approach. Monoclonal antibody reactivity was tested in vitro in ELISA, Western blot, and cytofluorimetry. Data confirmed that a panel of antibodies, reactive against shared idiotypes, can be produced from patients with HCV-associated B-cell lymphoproliferative diseases, thus obviating the need to produce an anti-idiotype antibody for each patient.
Subjects 1300 Biochemistry, Genetics and Molecular Biology
Keyword HCV
Idiotype
Lymphoma
Monoclonal antibody
NHL
Single-chain immunoglobulin variable fragment
Type II mixed cryoglobulinemia
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

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