Protein expression profile of celiac disease patient with aberrant T cell by two-dimensional difference gel electrophoresis

De Re, Valli, Simula, Maria Paola, Caggiari, Laura, Ortz, Nicoletta, Spina, Michele, Da Ponte, Alessandro, De Appolonia, Leandro, Dolcetti, Riccardo, Canzonieri, Vincenzo and Cannizzaro, Renato (2007). Protein expression profile of celiac disease patient with aberrant T cell by two-dimensional difference gel electrophoresis. In: , , (429-440). . doi:10.1196/annals.1398.049


Author De Re, Valli
Simula, Maria Paola
Caggiari, Laura
Ortz, Nicoletta
Spina, Michele
Da Ponte, Alessandro
De Appolonia, Leandro
Dolcetti, Riccardo
Canzonieri, Vincenzo
Cannizzaro, Renato
Title of paper Protein expression profile of celiac disease patient with aberrant T cell by two-dimensional difference gel electrophoresis
Journal name Autoimmunity, Part A: Basic Principles and New Diagnostic Tools   Check publisher's open access policy
Publication Year 2007
Sub-type Fully published paper
DOI 10.1196/annals.1398.049
ISBN 1573316636
ISSN 0077-8923
Volume 1109
Start page 429
End page 440
Total pages 12
Language eng
Abstract/Summary One complication of celiac disease (CD) is refractory CD. These patients frequently show aberrant intraepithelial T cell clones and an increasing risk of evolution into enteropathy-associated T cell lymphoma (EATL). There is debate in the literature whether these cases are actually a smoldering lymphoma from the outset. The mechanism inducing T cell proliferation and prognosis remains unknown. Recently, alemtuzumab has been proposed as a promising new approach to treat these patients. Only few single cases have been tested presently, nevertheless, in all of them a clinical improvement has been observed, while intraepithelial lymphocytes (IELs) effectively targeted by alemtuzumab are still a debated issue. Using 2D-DIGE, we found hyperexpressed proteins specifically associated with aberrant T cell in a patient with CD by comparing the protein expression with that of patients with CD and polyclonal T cell or with that of control subjects (patients with polyclonal T cell and no CD). Proteins with a higher expression in duodenal biopsy of the patient with aberrant T cell were identified as IgM, apolipoprotein C-III, and Charcot-Leyden crystal proteins. These preliminary data allow hypothesizing different clinical effects of alemtuzumab in patients with CD, since besides the probable effect of alemtuzumab on T cell, it could effect inflammatory-associated CD52+ IgM+ B cell and eosinophils cells, known to produce IgM and Charcot-Leyden crystal proteins, which we demonstrated to be altered in this patient. Results also emphasize the possible association of apolipoprotein with aberrant T cell proliferation.
Subjects 1300 Biochemistry, Genetics and Molecular Biology
Keyword 2D-DIGE
Celiac disease
Enteropathy-associated T cell lymphoma
T cell receptor
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
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