Specific antibodies reacting with simian virus 40 capsid protein mimotopes in serum samples from healthy blood donors

Corallini, Alfredo, Mazzoni, Elisa, Taronna, Angelo, Manfrini, Marco, Carandina, Giovanni, Guerra, Giovanni, Guaschino, Roberto, Vaniglia, Francesca, Magnani, Corrado, Casali, Ferruccio, Dolcetti, Riccardo, Palmonari, Caterina, Rezza, Giovanni, Martini, Fernanda, Barbanti-Brodano, Giuseppe and Tognon, Mauro G. (2012) Specific antibodies reacting with simian virus 40 capsid protein mimotopes in serum samples from healthy blood donors. Human Immunology, 73 5: 502-510. doi:10.1016/j.humimm.2012.02.009


Author Corallini, Alfredo
Mazzoni, Elisa
Taronna, Angelo
Manfrini, Marco
Carandina, Giovanni
Guerra, Giovanni
Guaschino, Roberto
Vaniglia, Francesca
Magnani, Corrado
Casali, Ferruccio
Dolcetti, Riccardo
Palmonari, Caterina
Rezza, Giovanni
Martini, Fernanda
Barbanti-Brodano, Giuseppe
Tognon, Mauro G.
Title Specific antibodies reacting with simian virus 40 capsid protein mimotopes in serum samples from healthy blood donors
Journal name Human Immunology   Check publisher's open access policy
ISSN 0198-8859
1879-1166
Publication date 2012-05
Year available 2012
Sub-type Article (original research)
DOI 10.1016/j.humimm.2012.02.009
Open Access Status Not yet assessed
Volume 73
Issue 5
Start page 502
End page 510
Total pages 9
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Abstract Simian virus 40 (SV40), a small DNA tumor virus, was inadvertently administered to human populations with the use of contaminated vaccines. SV40 sequences have mainly been detected in healthy individuals and cancer patients using polymerase chain reaction techniques. However, some studies have failed to reveal the presence of SV40 in human specimens. These conflicting results indicate the need for new research to verify whether SV40 is circulating in humans. Mimotopes from SV40 structural peptides were tested to investigate for specific reactions to human sera antibodies. An indirect enzyme-linked immunosorbent assay with synthetic peptides from SV40 viral capsid proteins 1-2-3 (VPs 1-2-3) was set up and employed to test 855 serum samples from healthy blood donors. Data from immunologic assays indicate that serum antibodies against SV40 VP mimotopes are detectable, although with a low titer, in blood donors 18 to 65 years old. The overall prevalence of serum samples that reacted with the 2 SV40 VP peptides was 18%. The strong points for this novel method include the simplicity of its approach and the potential to discriminate between SV40-specific antibody responses and to draw correlations between responses to the 2 independent SV40 peptides. These data suggest that SV40, or a yet undetected closely related polyomavirus, is circulating in human populations, but with lower prevalence than that of the ubiquitous BK and JC human polyomaviruses.
Keyword Donor
ELISA
Epitope
Mimotope
Serum
SV40
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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