Wolbachia-mediated antiviral protection in Drosophila larvae and adults following oral infection

Stevanovic, Aleksej L., Arnold, Pieter A. and Johnson, Karyn N. (2015) Wolbachia-mediated antiviral protection in Drosophila larvae and adults following oral infection. Applied and Environmental Microbiology, 81 23: 8215-8223. doi:10.1128/AEM.02841-15

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Author Stevanovic, Aleksej L.
Arnold, Pieter A.
Johnson, Karyn N.
Title Wolbachia-mediated antiviral protection in Drosophila larvae and adults following oral infection
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 1098-5336
Publication date 2015-12-01
Year available 2015
Sub-type Article (original research)
DOI 10.1128/AEM.02841-15
Open Access Status File (Publisher version)
Volume 81
Issue 23
Start page 8215
End page 8223
Total pages 9
Place of publication Washington, DC United States
Publisher American Society for Microbiology
Collection year 2016
Language eng
Formatted abstract
Understanding viral dynamics in arthropods is of great importance when designing models to describe how viral spread can influence arthropod populations. The endosymbiotic bacterium Wolbachia spp., which is present in up to 40% of all insect species, has the ability to alter viral dynamics in both Drosophila spp. and mosquitoes, a feature that in mosquitoes may be utilized to limit spread of important arboviruses. To understand the potential effect of Wolbachia on viral dynamics in nature, it is important to consider the impact of natural routes of virus infection on Wolbachia antiviral effects. Using adult Drosophila strains, we show here that Drosophila-Wolbachia associations that have previously been shown to confer antiviral protection following systemic viral infection also confer protection against virus-induced mortality following oral exposure to Drosophila C virus in adults. Interestingly, a different pattern was observed when the same fly lines were challenged with the virus when still larvae. Analysis of the four Drosophila-Wolbachia associations that were protective in adults indicated that only the w1118-wMelPop association conferred protection in larvae following oral delivery of the virus. Analysis of Wolbachia density using quantitative PCR (qPCR) showed that a high Wolbachia density was congruent with antiviral protection in both adults and larvae. This study indicates that Wolbachia-mediated protection may vary between larval and adult stages of a given Wolbachia-host combination and that the variations in susceptibility by life stage correspond with Wolbachia density. The differences in the outcome of virus infection are likely to influence viral dynamics in Wolbachia-infected insect populations in nature and could also have important implications for the transmission of arboviruses in mosquito populations.
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Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biological Sciences Publications
 
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