Distribution of technetium-99m PEG-liposomes during oligofructose-induced laminitis development in horses

Underwood, Claire, Pollitt, Christopher C., Metselaar, Josbert M., Laverman, Peter, van Bloois, Louis, van den Hoven, Jolanda m., Storm, Gert and van Eps, Andrew W. (2015) Distribution of technetium-99m PEG-liposomes during oligofructose-induced laminitis development in horses. Veterinary Journal, 206 2: 218-225. doi:10.1016/j.tvjl.2015.07.013

Author Underwood, Claire
Pollitt, Christopher C.
Metselaar, Josbert M.
Laverman, Peter
van Bloois, Louis
van den Hoven, Jolanda m.
Storm, Gert
van Eps, Andrew W.
Title Distribution of technetium-99m PEG-liposomes during oligofructose-induced laminitis development in horses
Journal name Veterinary Journal   Check publisher's open access policy
ISSN 1532-2971
Publication date 2015-11-01
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.tvjl.2015.07.013
Open Access Status Not yet assessed
Volume 206
Issue 2
Start page 218
End page 225
Total pages 8
Place of publication London, United Kingdom
Publisher Elsevier
Collection year 2016
Language eng
Formatted abstract
Liposomes are phospholipid nanoparticles used for targeted drug delivery. This study aimed to determine whether intravenous liposomes accumulate in lamellar tissue during laminitis development in horses so as to assess their potential for targeted lamellar drug delivery. Polyethylene-glycol (PEG) coated liposomes were prepared according to the film hydration method and labelled using 99mTc-hexamethyl-propylene-amine-oxime. Six horses received 10 g/kg oligofructose via nasogastric tube to induce laminitis, and four control horses received water via nasogastric tube. All horses received 300 µmol 99mTc-PEG-liposomes (5.5 GBq) plus 5.5 µmol/kg PEG-liposomes by slow intravenous infusion. Scintigraphic imaging was performed at 0, 6 and 12 h post-infusion. Technetium-99m liposome uptake was measured in regions of interest over the hoof, fetlock and metacarpus. At the study end-point horses were euthanased, tissue samples collected and tissue liposome levels were calculated as the percentage of the injected dose of 99mTc-liposomes per kilogram of tissue. Data were analysed non-parametrically.

All horses receiving oligofructose developed clinical and histological signs of laminitis. Technetium-99m liposome uptake in the hoof increased with time in laminitis horses (P = 0.04), but decreased with time in control horses (P = 0.01). Technetium-99m liposome levels in lamellar tissue from laminitis horses were 3.2-fold higher than controls (P = 0.02) and were also higher in laminitis vs. control skin, muscle, jejunum, colon, and kidney (P < 0.05). Liposomes accumulated in lamellar tissue during oligofructose-induced laminitis development and demonstrated potential for targeted lamellar drug delivery in acute laminitis. This study provides further evidence that lamellar inflammation occurs during laminitis development. Liposome accumulation also occurred in the skin, muscle, jejunum, colon and kidneys, suggesting systemic inflammation in this model.
Keyword Nanoparticle
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Veterinary Science Publications
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