Nf-protocadherin regulates retinal ganglion cell axon behaviour in the developing visual system

Leung, Louis C., Harris, William A., Holt, Christine E. and Piper, Michael (2015) Nf-protocadherin regulates retinal ganglion cell axon behaviour in the developing visual system. Plos One, 10 10: 1-17. doi:10.1371/journal.pone.0141290


Author Leung, Louis C.
Harris, William A.
Holt, Christine E.
Piper, Michael
Title Nf-protocadherin regulates retinal ganglion cell axon behaviour in the developing visual system
Journal name Plos One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2015-10
Sub-type Article (original research)
DOI 10.1371/journal.pone.0141290
Open Access Status DOI
Volume 10
Issue 10
Start page 1
End page 17
Total pages 17
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2016
Language eng
Abstract Cell adhesion molecules play a central role in mediating axonal tract development within the nascent nervous system. NF-protocadherin (NFPC), a member of the non-clustered protocadherin family, has been shown to regulate retinal ganglion cell (RGC) axon and dendrite initiation, as well as influencing axonal navigation within the mid-optic tract. However, whether NFPC mediates RGC axonal behaviour at other positions within the optic pathway remains unclear. Here we report that NFPC plays an important role in RGC axonogenesis, but not in intraretinal guidance. Moreover, axons with reduced NFPC levels exhibit insensitivity to Netrin-1, an attractive guidance cue expressed at the optic nerve head. Netrin-1 induces rapid turnover of NFPC localized to RGC growth cones, suggesting that the regulation of NFPC protein levels may underlie Netrin-1-mediated entry of RGC axons into the optic nerve head. At the tectum, we further reveal a function for NFPC in controlling RGC axonal entry into the final target area. Collectively, our results expand our understanding of the role of NFPC in RGC guidance and illustrate that this adhesion molecule contributes to axon behaviour at multiple points in the optic pathway.
Keyword Growth cones
In-vivo
Chemotropic responses
Cadherin superfamily
Gamma-protocadherins
Protein-synthesis
Time-lapse
Guidance
Xenopus
Netrin-1
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2016 Collection
School of Biomedical Sciences Publications
 
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