The role of H4 flagella in Escherichia coli ST131 virulence

Kakkanat, Asha, Totsika, Makrina, Schaale, Kolja, Duell, Benjamin L., Lo, Alvin W., Phan, Minh-Duy, Moriel, Danilo G., Beatson, Scott A., Sweet, Matthew J., Ulett, Glen C. and Schembri, Mark A. (2015) The role of H4 flagella in Escherichia coli ST131 virulence. Scientific Reports, 5 16149: 1-14. doi:10.1038/srep16149


Author Kakkanat, Asha
Totsika, Makrina
Schaale, Kolja
Duell, Benjamin L.
Lo, Alvin W.
Phan, Minh-Duy
Moriel, Danilo G.
Beatson, Scott A.
Sweet, Matthew J.
Ulett, Glen C.
Schembri, Mark A.
Title The role of H4 flagella in Escherichia coli ST131 virulence
Formatted title
The role of H4 flagella in Escherichia coli ST131 virulence
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2015-11-09
Year available 2015
Sub-type Article (original research)
DOI 10.1038/srep16149
Open Access Status DOI
Volume 5
Issue 16149
Start page 1
End page 14
Total pages 14
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2016
Language eng
Formatted abstract
Escherichia coli sequence type 131 (ST131) is a globally dominant multidrug resistant clone associated with urinary tract and bloodstream infections. Most ST131 strains exhibit resistance to multiple antibiotics and cause infections associated with limited treatment options. The largest sub-clonal ST131 lineage is resistant to fluoroquinolones, contains the type 1 fimbriae fimH30 allele and expresses an H4 flagella antigen. Flagella are motility organelles that contribute to UPEC colonisation of the upper urinary tract. In this study, we examined the specific role of H4 flagella in ST131 motility and interaction with host epithelial and immune cells. We show that the majority of H4-positive ST131 strains are motile and are enriched for flagella expression during static pellicle growth. We also tested the role of H4 flagella in ST131 through the construction of specific mutants, over-expression strains and isogenic mutants that expressed alternative H1 and H7 flagellar subtypes. Overall, our results revealed that H4, H1 and H7 flagella possess conserved phenotypes with regards to motility, epithelial cell adhesion, invasion and uptake by macrophages. In contrast, H4 flagella trigger enhanced induction of the anti-inflammatory cytokine IL-10 compared to H1 and H7 flagella, a property that may contribute to ST131 fitness in the urinary tract.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 13 Nov 2015, 12:31:56 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences