Quantification of NS1 dengue biomarker in serum via optomagnetic nanocluster detection.

Antunes, Paula, Watterson, Daniel, Parmvi, Mattias, Burger, Robert, Boisen, Anja, Young, Paul, Cooper, Matthew A., Hansen, Mikkel F., Ranzoni, Andrea and Donolato, Marco (2015) Quantification of NS1 dengue biomarker in serum via optomagnetic nanocluster detection.. Scientific Reports, 5 16145: 1-10. doi:10.1038/srep16145

Author Antunes, Paula
Watterson, Daniel
Parmvi, Mattias
Burger, Robert
Boisen, Anja
Young, Paul
Cooper, Matthew A.
Hansen, Mikkel F.
Ranzoni, Andrea
Donolato, Marco
Title Quantification of NS1 dengue biomarker in serum via optomagnetic nanocluster detection.
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2015-11-05
Year available 2015
Sub-type Article (original research)
DOI 10.1038/srep16145
Open Access Status DOI
Volume 5
Issue 16145
Start page 1
End page 10
Total pages 10
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2016
Language eng
Abstract Dengue is a tropical vector-borne disease without cure or vaccine that progressively spreads into regions with temperate climates. Diagnostic tools amenable to resource-limited settings would be highly valuable for epidemiologic control and containment during outbreaks. Here, we present a novel low-cost automated biosensing platform for detection of dengue fever biomarker NS1 and demonstrate it on NS1 spiked in human serum. Magnetic nanoparticles (MNPs) are coated with high-affinity monoclonal antibodies against NS1 via bio-orthogonal Cu-free ‘click’ chemistry on an anti-fouling surface molecular architecture. The presence of the target antigen NS1 triggers MNP agglutination and the formation of nanoclusters with rapid kinetics enhanced by external magnetic actuation. The amount and size of the nanoclusters correlate with the target concentration and can be quantified using an optomagnetic readout method. The resulting automated dengue fever assay takes just 8 minutes, requires 6 μL of serum sample and shows a limit of detection of 25 ng/mL with an upper detection range of 20000 ng/mL. The technology holds a great potential to be applied to NS1 detection in patient samples. As the assay is implemented on a low-cost microfluidic disc the platform is suited for further expansion to multiplexed detection of a wide panel of biomarkers.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 13 Nov 2015, 11:50:28 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences