Analytical ultracentrifugation: A versatile tool for the characterisation of macromolecular complexes in solution.

Patel, Trushar R., Winzor, Donald J. and Scott, David J. (2015) Analytical ultracentrifugation: A versatile tool for the characterisation of macromolecular complexes in solution.. Methods, 95 55-61. doi:10.1016/j.ymeth.2015.11.006


Author Patel, Trushar R.
Winzor, Donald J.
Scott, David J.
Title Analytical ultracentrifugation: A versatile tool for the characterisation of macromolecular complexes in solution.
Journal name Methods   Check publisher's open access policy
ISSN 1046-2023
1095-9130
Publication date 2015-11-10
Year available 2015
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/j.ymeth.2015.11.006
Open Access Status Not Open Access
Volume 95
Start page 55
End page 61
Total pages 7
Place of publication Maryland Heights, MO United States
Publisher Academic Press
Collection year 2016
Language eng
Formatted abstract
Analytical ultracentrifugation, an early technique developed for characterizing quantitatively the solution properties of macromolecules, remains a powerful aid to structural biologists in their quest to understand the formation of biologically important protein complexes at the molecular level. Treatment of the basic tenets of the sedimentation velocity and sedimentation equilibrium variants of analytical ultracentrifugation is followed by considerations of the roles that it, in conjunction with other physicochemical procedures, has played in resolving problems encountered in the delineation of complex formation for three biological systems – the cytoplasmic dynein complex, mitogen-activated protein kinase (ERK2)
self-interaction, and the terminal catalytic complex in selenocysteine synthesis.
Keyword Analytical Ultracentrifugation
Catalytic complex in selenocysteine synthesis
Cytoplasmic dynein complex
Mitogen-activated protein kinase (ERK2)
Sedimentation equilibrium
Sedimentation velocityl
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 13 Nov 2015, 11:09:02 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences