Mucosal polyinosinic-polycytidylic acid improves protection elicited by replicating influenza vaccines via enhanced dendritic cell function and T cell immunity

Perez-Giron, Jose V., Belicha-Villanueva, Alan, Hassan, Ebrahim, Gomez-Medina, Sergio, Cruz, Jazmina L. G., Ludtke, Anja, Ruibal, Paula, Albrecht, Randy A., Garcia-Sastre, Adolfo and Munoz-Fontela, Cesar (2014) Mucosal polyinosinic-polycytidylic acid improves protection elicited by replicating influenza vaccines via enhanced dendritic cell function and T cell immunity. Journal of Immunology, 193 3: 1324-1332. doi:10.4049/jimmunol.1400222


Author Perez-Giron, Jose V.
Belicha-Villanueva, Alan
Hassan, Ebrahim
Gomez-Medina, Sergio
Cruz, Jazmina L. G.
Ludtke, Anja
Ruibal, Paula
Albrecht, Randy A.
Garcia-Sastre, Adolfo
Munoz-Fontela, Cesar
Title Mucosal polyinosinic-polycytidylic acid improves protection elicited by replicating influenza vaccines via enhanced dendritic cell function and T cell immunity
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
1550-6606
Publication date 2014-08-01
Year available 2014
Sub-type Article (original research)
DOI 10.4049/jimmunol.1400222
Open Access Status DOI
Volume 193
Issue 3
Start page 1324
End page 1332
Total pages 9
Place of publication Bethesda, MD United States
Publisher American Association of Immunologists
Collection year 2015
Language eng
Abstract Live-attenuated influenza vaccines (LAIVs) have the potential to generate CD8 T cell immunity that may limit the virulence of an antigenically shifted influenza strain in a population lacking protective Abs. However, current LAIVs exert limited T cell immunity restricted to the vaccine strains. One approach to improve LAIV-induced T cell responses is the use of specific adjuvants to enhance T cell priming by respiratory dendritic cells, but this hypothesis has not been addressed. In this study, we assessed the effect of the TLR3 ligand polyinosinic-polycytidylic acid (poly IC) on CD8 T cell immunity and protection elicited by LAIVs. Mucosal treatment with poly IC shortly after vaccination enhanced respiratory dendritic cell function, CD8 T cell formation, and production of neutralizing Abs. This adjuvant effect of poly IC was dependent on amplification of TLR3 signaling by nonhematopoietic radioresistant cells and enhanced mouse protection to homosubtypic, as well as heterosubtypic, virus challenge. Our findings indicate that mucosal TLR3 ligation may be used to improve CD8 T cell responses to replicating vaccines, which has implications for protection in the absence of pre-existing Ab immunity. Copyright
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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Created: Wed, 11 Nov 2015, 12:48:48 EST by Jazmina Gonzalez Cruz on behalf of UQ Diamantina Institute