Alphacoronavirus protein 7 modulates host innate immune response

Cruz, Jazmina L. G., Becares, Martina, Sola, Isabel, Oliveros, Juan Carlos, Enjuanes, Luis and Zuñiga, Sonia (2013) Alphacoronavirus protein 7 modulates host innate immune response. Journal of Virology, 87 17: 9754-9767. doi:10.1128/JVI.01032-13

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Author Cruz, Jazmina L. G.
Becares, Martina
Sola, Isabel
Oliveros, Juan Carlos
Enjuanes, Luis
Zuñiga, Sonia
Title Alphacoronavirus protein 7 modulates host innate immune response
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1070-6321
Publication date 2013-07-03
Sub-type Article (original research)
DOI 10.1128/JVI.01032-13
Open Access Status File (Publisher version)
Volume 87
Issue 17
Start page 9754
End page 9767
Total pages 14
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract Innate immune response is the first line of antiviral defense resulting, in most cases, in pathogen clearance with minimal clinical consequences. Viruses have developed diverse strategies to subvert host defense mechanisms and increase their survival. In the transmissible gastroenteritis virus (TGEV) as a model, we previously reported that accessorygene 7 counteracts the host antiviral response by associating with the catalytic subunit of protein phosphatase 1 (PP1c). In the present work, the effect of the absence of gene 7on the host cell, during infection, was further analyzed by transcriptomic analysis. The pattern of gene expression of cells infected with a recombinant mutant TGEV, lacking gene7 expression (rTGEV-δ7), was compared to that of cells infected with the parental virus (rTGEV-wt). Genes involved in the immune response, the interferon response, and inflammation were upregulated during TGEV infection in the absence of gene 7. An exacerbated innate immune response during infection with rTGEV-δ7 virus was observed both in vitro and invivo. An increase in macrophage recruitment and activation in lung tissues infected with rTGEV-δ7 virus was observed compared to cells infected with the parental virus. In summary, the absence of protein 7 both in vitro and in vivo led to increased proinflammatory responses and acute tissue damage after infection. In a porcine animal model, which is immunologically similar to humans, we present a novel example of how viral proteins counteract host antiviral pathways to determine the infection outcome and pathogenesis.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
 
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Created: Wed, 11 Nov 2015, 11:16:29 EST by Jazmina Gonzalez Cruz on behalf of UQ Diamantina Institute