Oral T4-like phage cocktail application to healthy adult volunteers from Bangladesh

Sarker, Shafiqul Alam, McCallin, Shawna, Barretto, Caroline, Berger, Bernard, Pittet, Anne-Cecile, Sultana, Shamima, Krause, Lutz, Huq, Sayeda, Bibiloni, Rodrigo, Bruttin, Anne, Reuteler, Gloria and Bruessow, Harald (2012) Oral T4-like phage cocktail application to healthy adult volunteers from Bangladesh. Virology, 434 2: 222-232. doi:10.1016/j.virol.2012.09.002


Author Sarker, Shafiqul Alam
McCallin, Shawna
Barretto, Caroline
Berger, Bernard
Pittet, Anne-Cecile
Sultana, Shamima
Krause, Lutz
Huq, Sayeda
Bibiloni, Rodrigo
Bruttin, Anne
Reuteler, Gloria
Bruessow, Harald
Title Oral T4-like phage cocktail application to healthy adult volunteers from Bangladesh
Journal name Virology   Check publisher's open access policy
ISSN 0042-6822
1089-862X
Publication date 2012-12-20
Sub-type Article (original research)
DOI 10.1016/j.virol.2012.09.002
Open Access Status Not Open Access
Volume 434
Issue 2
Start page 222
End page 232
Total pages 11
Place of publication Waltham, MA, United States
Publisher Academic Press
Language eng
Abstract The genomic diversity of 99 T4-like coliphages was investigated by sequencing an equimolar mixture with Illumina technology and screening them against different databases for horizontal gene transfer and undesired genes. A 9-phage cocktail was given to 15 healthy adults from Bangladesh at a dose of 3×109 and 3×107 plaque-forming units and placebo respectively. Phages were detected in 64% of the stool samples when subjects were treated with higher titer phage, compared to 30% and 28% with lower-titer phage and placebo, respectively. No Escherichia coli was present in initial stool samples, and no amplification of phage was observed. One percent of the administered oral phage was recovered from the feces. No adverse events were observed by self-report, clinical examination, or from laboratory tests for liver, kidney, and hematology function. No impact of oral phage was seen on the fecal microbiota composition with respect to bacterial 16S rRNA from stool.
Keyword Bacteriophages
Bioavailability
Escherichia coli
Fecal microbiota
Human phage therapy
Safety
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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