Smchd1 regulates a subset of autosomal genes subject to monoallelic expression in addition to being critical for X inactivation

Mould, Arne W., Pang, Zhenyi, Pakusch, Miha, Tonks, Ian D., Stark, Mitchell, Carrie, Dianne, Mukhopadhyay, Pamela, Seidel, Annica, Ellis, Jonathan J., Deakin, Janine, Wakefield, Matthew J., Krause, Lutz, Blewitt, Marnie E. and Kay, Graham F. (2013) Smchd1 regulates a subset of autosomal genes subject to monoallelic expression in addition to being critical for X inactivation. Epigenetics and Chromatin, 6 19: 1-16. doi:10.1186/1756-8935-6-19


Author Mould, Arne W.
Pang, Zhenyi
Pakusch, Miha
Tonks, Ian D.
Stark, Mitchell
Carrie, Dianne
Mukhopadhyay, Pamela
Seidel, Annica
Ellis, Jonathan J.
Deakin, Janine
Wakefield, Matthew J.
Krause, Lutz
Blewitt, Marnie E.
Kay, Graham F.
Title Smchd1 regulates a subset of autosomal genes subject to monoallelic expression in addition to being critical for X inactivation
Journal name Epigenetics and Chromatin   Check publisher's open access policy
ISSN 1756-8935
1098-1004
Publication date 2013-07-02
Sub-type Article (original research)
DOI 10.1186/1756-8935-6-19
Open Access Status DOI
Volume 6
Issue 19
Start page 1
End page 16
Total pages 16
Place of publication Hoboken, NJ United States
Publisher John Wiley & Sons
Language eng
Formatted abstract
Background
Smchd1 is an epigenetic modifier essential for X chromosome inactivation: female embryos lacking Smchd1 fail during midgestational development. Male mice are less affected by Smchd1-loss, with some (but not all) surviving to become fertile adults on the FVB/n genetic background. On other genetic backgrounds, all males lacking Smchd1 die perinatally. This suggests that, in addition to being critical for X inactivation, Smchd1 functions to control the expression of essential autosomal genes.

Results
Using genome-wide microarray expression profiling and RNA-seq, we have identified additional genes that fail X inactivation in female Smchd1 mutants and have identified autosomal genes in male mice where the normal expression pattern depends upon Smchd1. A subset of genes in the Snrpn imprinted gene cluster show an epigenetic signature and biallelic expression consistent with loss of imprinting in the absence of Smchd1. In addition, single nucleotide polymorphism analysis of expressed genes in the placenta shows that the Igf2r imprinted gene cluster is also disrupted, with Slc22a3 showing biallelic expression in the absence of Smchd1. In both cases, the disruption was not due to loss of the differential methylation that marks the imprint control region, but affected genes remote from this primary imprint controlling element. The clustered protocadherins (Pcdhα, Pcdhβ, and Pcdhγ) also show altered expression levels, suggesting that their unique pattern of random combinatorial monoallelic expression might also be disrupted.

Conclusions
Smchd1 has a role in the expression of several autosomal gene clusters that are subject to monoallelic expression, rather than being restricted to functioning uniquely in X inactivation. Our findings, combined with the recent report implicating heterozygous mutations of SMCHD1 as a causal factor in the digenically inherited muscular weakness syndrome facioscapulohumeral muscular dystrophy-2, highlight the potential importance of Smchd1 in the etiology of diverse human diseases.
Keyword Clustered protocadherins
Genomic imprinting
Monoallelic expression
Smchd1
X inactivation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Medicine Publications
UQ Diamantina Institute Publications
 
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