Genomic sequence of chorioallantois vaccinia virus Ankara, the ancestor of modified vaccinia virus Ankara

Meisinger-Henschel, Christine, Schmidt, Michaela, Lukassen, Susanne, Linke, Burkhard, Krause, Lutz, Konietzny, Sebastian, Goesmann, Alexander, Howley, Paul, Chaplin, Paul, Suter, Mark and Hausmann, Juergen (2007) Genomic sequence of chorioallantois vaccinia virus Ankara, the ancestor of modified vaccinia virus Ankara. Journal of General Virology, 88 12: 3249-3259. doi:10.1099/vir.0.83156-0

Author Meisinger-Henschel, Christine
Schmidt, Michaela
Lukassen, Susanne
Linke, Burkhard
Krause, Lutz
Konietzny, Sebastian
Goesmann, Alexander
Howley, Paul
Chaplin, Paul
Suter, Mark
Hausmann, Juergen
Title Genomic sequence of chorioallantois vaccinia virus Ankara, the ancestor of modified vaccinia virus Ankara
Journal name Journal of General Virology   Check publisher's open access policy
ISSN 0022-1317
Publication date 2007-12
Sub-type Article (original research)
DOI 10.1099/vir.0.83156-0
Open Access Status Not yet assessed
Volume 88
Issue 12
Start page 3249
End page 3259
Total pages 11
Place of publication London, United Kingdom
Publisher The Microbiology Society
Language eng
Abstract Chorioallantois vaccinia virus Ankara (CVA) is the parental virus of modified vaccinia virus Ankara (MVA), which was derived from CVA by more than 570 passages in chicken embryo fibroblasts (CEF). MVA became severely host-cell-restricted to avian cells and has strongly diminished virulence in mammalian hosts, while maintaining good immunogenicity. We determined the complete coding sequence of the parental CVA and mapped the exact positions of the six major deletions that emerged in the MVA genome. All six major deletions occurred in regions of the CVA genome where one or more truncated or fragmented open reading frames (ORFs) pre-existed. The CVA genome contains 229 ORFs of which 51 are fragments of full-length orthopoxvirus (OPV) genes, including fragmented orthologues of C9L and M1L (encoding two well-conserved ankyrin-like proteins), A39R (encoding a semaphorin-like protein) and A55R (encoding a kelch-like protein). Phylogenetic analysis demonstrated that MVA was most closely related to CVA, followed by the vaccinia virus (VACV) strain DUKE, a patient-derived isolate of the Dryvax vaccine virus. Loss or mutation of genes outside the six major deletions are assumed to contribute to the restricted host range phenotype of MVA. In support of this notion, deletions, insertions and non-synonymous mutations were found in 122 of the 195 ORFs remaining in MVA when compared with their CVA counterparts. Thus, detailed knowledge of the CVA genomic sequence is a prerequisite to further dissect the genetic basis of the MVA host range phenotype as well as the particular immunological properties of MVA.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
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