Treatment of licorice seeds with colchicine: changes in seedling DNA levels and anthocyanin and glycyrrhizic acid contents of derived callus cultures

Bernard, Françoise, Moghbel, Nahid and Hassannejad, Sahar (2012) Treatment of licorice seeds with colchicine: changes in seedling DNA levels and anthocyanin and glycyrrhizic acid contents of derived callus cultures. Natural Product Communications, 7 11: 1457-1460.

Author Bernard, Françoise
Moghbel, Nahid
Hassannejad, Sahar
Title Treatment of licorice seeds with colchicine: changes in seedling DNA levels and anthocyanin and glycyrrhizic acid contents of derived callus cultures
Journal name Natural Product Communications   Check publisher's open access policy
ISSN 1934-578X
1555-9475
Publication date 2012-07
Sub-type Article (original research)
Open Access Status Not Open Access
Volume 7
Issue 11
Start page 1457
End page 1460
Total pages 4
Place of publication Westerville, OH, United States
Publisher Natural Product
Language eng
Abstract The use of colchicine to induce polyploids increases secondary metabolite production potential and has been used for many years for the production of valuable compounds in plants. This project took advantage of this method to increase the production of secondary metabolites in licorice. For this purpose, seeds of licorice, Glycyrrhiza glabra var. glandulifera, were treated with different concentrations of colchicine for 24 hours and then cultivated in vitro. After a month, the effect of colchicine on the cellular DNA level of cotyledons was analyzed by spectrophotometry and flow cytometry. For callus induction, root explants of one month old plantlets derived from colchicine treated seeds were transferred to MS medium containing growth regulators and the anthocyanin and glycyrrhizic acid levels of the callus tissues were measured after two months of growth. The total DNA content of plantlets derived from seeds treated with 0.05%, 0.08% and 0.1% of colchicine for 24 hours was increased significantly. Treated plants had increased numbers of larger stomata, significantly in those treated with 0.1% of colchicine for 24 hours. After colchicine treatment, the root, shoot and leaf thickness was found to be increased, while their length was decreased. Results of flow cytometry showed changes in ploidy level in plantlets obtained from treatment with 0.08% (mixoploids) and 0.1% (tetraploids) of colchicine. Anthocyanin level was significantly increased in callus obtained from plantlets treated with 0.08% of colchicine. The amount of glycyrrhizic acid in all treatments increased, especially in the 0.1 and 0.03% colchicine treatments and this seems to prove an increased production of metabolites in polyploid licorice tissues.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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Created: Thu, 29 Oct 2015, 17:06:16 EST by Nahid Moghbel on behalf of School of Pharmacy