Peyronie's disease and mechanotransduction: an in vitro analysis of the cellular changes to Peyronie's disease in a cell-culture strain system

Chung, Eric, De Young, Ling, Solomon, Matthew and Brock, Gerald B. (2013) Peyronie's disease and mechanotransduction: an in vitro analysis of the cellular changes to Peyronie's disease in a cell-culture strain system. Journal of Sexual Medicine, 10 5: 1259-1267. doi:10.1111/jsm.12082


Author Chung, Eric
De Young, Ling
Solomon, Matthew
Brock, Gerald B.
Title Peyronie's disease and mechanotransduction: an in vitro analysis of the cellular changes to Peyronie's disease in a cell-culture strain system
Journal name Journal of Sexual Medicine   Check publisher's open access policy
ISSN 1743-6095
1743-6109
Publication date 2013-05
Year available 2013
Sub-type Article (original research)
DOI 10.1111/jsm.12082
Open Access Status Not yet assessed
Volume 10
Issue 5
Start page 1259
End page 1267
Total pages 9
Place of publication Oxford, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
Introduction: Penile traction therapy (PTT) has gained considerable popularity as a noninvasive treatment option in Peyronie's disease (PD) to improve penile curvature and length. However the exact mechanism of action of PTT is unknown and as yet unproven.

Aim: Using a cyclic strain culture system, we investigate the cellular changes to the tunical tissues following equibiaxial tractional forces. The observed protein alteration following mechanical traction should replicate the changes observed in tunical tissue following PTT.

Methods: FlexcellTM FX-5000TM Tension Plus system (FlexcellTM International Corporation, McKee-sport, PA, USA) is a pressurized chamber system that allows for sustained and dynamic application of hydrostatic pressure to cellular culture. Primary cell cultures from PD and normal tunical tissue (control) were sub-cultured on BioFlex-ProNectin plate 1×106 (Flexcell International Corporation, Hillsborough, NC, USA) for 24 hours and exposed to FlexcellTM sinusoidal strain of 18% at 1Hz (strained group) on the biaxial plates for 24 hours under 37°C in a 5% CO2 atmosphere.

Main Outcomes Measures: Immunohistochemistry (IHC) stains and Western blot assays between strained and nonstrained (control) cells. Results: Significant increase in smooth muscle α-actin, β-catenin, and Hsp 47 proteins were measured in the PD group compared with control group. IHC staining revealed a decrease in α-actin staining while Western blot assay showed an increase in metalloproteinase-8 expression with no measured change in β-catenin level in the strained PD group.

Conclusions: This novel study of cells derived from diseased and normal human tunica albuginea cultured in a mechanical strained environment provides good scientific evidence for the use of penile traction device in Peyronie's plaque remodeling.
Keyword Cellular strain model
Mechanotransduction
Penile curvature and length
Penile traction therapy
Peyronie's disease
Plaque remodeling
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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