Stepwise evolution of pandrug-resistance in Klebsiella pneumoniae.

Zowawi, Hosam M., Forde, Brian M., Alfaresi, Mubarak, Alzarouni, Abdulqadir, Farahat, Yasser, Chong, Teik-Min, Yin, Wai-Fong, Chan, Kok-Gan, Li, Jian, Schembri, Mark A., Beatson, Scott A. and Paterson, David L. (2015) Stepwise evolution of pandrug-resistance in Klebsiella pneumoniae.. Scientific Reports, 5 Art No.: 15082: . doi:10.1038/srep15082


Author Zowawi, Hosam M.
Forde, Brian M.
Alfaresi, Mubarak
Alzarouni, Abdulqadir
Farahat, Yasser
Chong, Teik-Min
Yin, Wai-Fong
Chan, Kok-Gan
Li, Jian
Schembri, Mark A.
Beatson, Scott A.
Paterson, David L.
Title Stepwise evolution of pandrug-resistance in Klebsiella pneumoniae.
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2015-10-19
Year available 2015
Sub-type Article (original research)
DOI 10.1038/srep15082
Open Access Status DOI
Volume 5
Issue Art No.: 15082
Total pages 8
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2016
Language eng
Formatted abstract
Carbapenem resistant Enterobacteriaceae (CRE) pose an urgent risk to global human health. CRE that are non-susceptible to all commercially available antibiotics threaten to return us to the preantibiotic era. Using Single Molecule Real Time (SMRT) sequencing we determined the complete genome of a pandrug-resistant Klebsiella pneumoniae isolate, representing the first complete genome sequence of CRE resistant to all commercially available antibiotics. The precise location of acquired antibiotic resistance elements, including mobile elements carrying genes for the OXA-181 carbapenemase, were defined. Intriguingly, we identified three chromosomal copies of an ISEcp1-blaOXA-181 mobile element, one of which has disrupted the mgrB regulatory gene, accounting for resistance to colistin. Our findings provide the first description of pandrug-resistant CRE at the genomic level, and reveal the critical role of mobile resistance elements in accelerating the emergence of resistance to other last resort antibiotics. 
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Fri, 23 Oct 2015, 10:34:53 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences