3' and 5' microRNA-end post-biogenesis modifications in plant transcriptomes: Evidences from small RNA next generation sequencing data analysis

Saraf, Shradha, Sanan-Mishra, Neeti, Gursanscky, Nial R., Carroll, Bernard J., Gupta, Dinesh and Mukherjee, Sunil Kumar (2015) 3' and 5' microRNA-end post-biogenesis modifications in plant transcriptomes: Evidences from small RNA next generation sequencing data analysis. Biochemical and Biophysical Research Communications, 467 4: 892-899. doi:10.1016/j.bbrc.2015.10.048

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Author Saraf, Shradha
Sanan-Mishra, Neeti
Gursanscky, Nial R.
Carroll, Bernard J.
Gupta, Dinesh
Mukherjee, Sunil Kumar
Title 3' and 5' microRNA-end post-biogenesis modifications in plant transcriptomes: Evidences from small RNA next generation sequencing data analysis
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
1090-2104
Publication date 2015-11
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2015.10.048
Open Access Status Not Open Access
Volume 467
Issue 4
Start page 892
End page 899
Total pages 51
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Collection year 2016
Language eng
Formatted abstract
The processing of miRNA from its precursors is a precisely regulated process and after biogenesis, the miRNAs are amenable to different kinds of modifications by the addition or deletion of nucleotides at the terminal ends. However, the mechanism and functions of such modifications are not well studied in plants. In this study, we have specifically analysed the terminal end non-templated miRNA modifications, using NGS data of rice, tomato and Arabidopsis small RNA transcriptomes from different tissues and physiological conditions. Our analysis reveals template independent terminal end modifications in the mature as well as passenger strands of the miRNA duplex. Interestingly, it is also observed that miRNA sequences terminating with a cytosine (C) at the 3′ end undergo a higher percentage of 5′ end modifications. The terminal end modifications did not correlate with the miRNA abundances and are independent of tissue types, physiological conditions and plant species. Our analysis indicates that the addition of nucleotides at miRNA ends is not influenced by the absence of RNA dependent RNA polymerase 6. Moreover the terminal end modified miRNAs are also observed amongst AGO1 bound small RNAs and have potential to alter target, indicating its important functional role in repression of gene expression.
Keyword AGO1
Modifications
Next generation sequencing
RDR6
miRNA
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 23 Oct 2015, 09:33:04 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences