Estimating the prevalence of mixed-type gonococcal infections in Queensland, Australia

Trembizki, Ella, Doyle, Christine, Buckley, Cameron, Jennison, Amy, Smith, Helen, Bates, John, Sloots, Theo, Nissen, Michael, Lahra, Monica M. and Whiley, David (2015) Estimating the prevalence of mixed-type gonococcal infections in Queensland, Australia. Sexual Health, 12 5: 439-444. doi:10.1071/SH15009


Author Trembizki, Ella
Doyle, Christine
Buckley, Cameron
Jennison, Amy
Smith, Helen
Bates, John
Sloots, Theo
Nissen, Michael
Lahra, Monica M.
Whiley, David
Title Estimating the prevalence of mixed-type gonococcal infections in Queensland, Australia
Journal name Sexual Health   Check publisher's open access policy
ISSN 1449-8987
Publication date 2015-07-06
Year available 2015
Sub-type Article (original research)
DOI 10.1071/SH15009
Open Access Status Not Open Access
Volume 12
Issue 5
Start page 439
End page 444
Total pages 6
Place of publication Collingwood, Victoria, Australia
Publisher CSIRO Publishing
Collection year 2016
Language eng
Formatted abstract
Background: Mixed gonococcal infections within the one anatomical site have been recognised but questions remain over how often they occur. In this study, the aim was to estimate the prevalence of mixed gonococcal infections using novel real-time polymerase chain reaction (PCR) methods that were developed and validated, targeting the gonococcal porB gene. Methods: Neisseria gonorrhoeae strains were categorised into three different porB groups, based on sequence data derived from N. gonorrhoeae multi-antigen sequence typing (NG-MAST) analyses of local isolates. Specific PCR methods for each group were then developed and these PCR methods were used to test clinical samples (n = 350) that were positive for gonorrhoea as determined by nucleic acid amplification test (NAAT) diagnostic screening. Results: Initial validation using isolates showed the group PCR methods proved 100% sensitive and 100% specific for their respective porB groups. When applied to the clinical specimens, 298/350 (85%) provided positive results by the group PCR methods. Of these, four specimens showed evidence of mixed infections, supported by subsequent DNA sequencing of the PCR products. Conclusions: The data provide further evidence of mixed gonococcal infections at the same anatomical site, but show that such infections may be relatively infrequent (1.3%; 95% confidence interval 0.01–2.6%) in a general screening population.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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