Xenopus borealis as an alternative source of oocytes for biophysical and pharmacological studies of neuronal ion channels

Cristofori-Armstrong, Ben, Soh, Ming S., Talwar, Sahil, Brown, Darren L., Griffin, John D. O., Dekan, Zoltan, Stow, Jennifer L., King, Glenn F., Lynch, Joseph W. and Rash, Lachlan D. (2015) Xenopus borealis as an alternative source of oocytes for biophysical and pharmacological studies of neuronal ion channels. Scientific Reports, 5 14763.1-14763.12. doi:10.1038/srep14763


Author Cristofori-Armstrong, Ben
Soh, Ming S.
Talwar, Sahil
Brown, Darren L.
Griffin, John D. O.
Dekan, Zoltan
Stow, Jennifer L.
King, Glenn F.
Lynch, Joseph W.
Rash, Lachlan D.
Title Xenopus borealis as an alternative source of oocytes for biophysical and pharmacological studies of neuronal ion channels
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2015-10-06
Sub-type Article (original research)
DOI 10.1038/srep14763
Open Access Status DOI
Volume 5
Start page 14763.1
End page 14763.12
Total pages 12
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2015
Language eng
Formatted abstract
For the past 30 years, oocytes from Xenopus laevis have been extensively used to express and characterise ion channels in an easily controlled environment. Here we report the first use of oocytes from the closely related species Xenopus borealis as an alternative expression system for neuronal ion channels. Using the two-electrode voltage-clamp technique, we show that a wide variety of voltage- and ligand-gated ion channels have the same channel properties and pharmacological profiles when expressed in either X. laevis or X. borealis oocytes. Potential advantages of the X. borealis oocytes include a smaller endogenous chloride current and the ability to produce more intense fluorescence signals when studied with voltage-clamp fluorometry. Scanning electron microscopy revealed a difference in vitelline membrane structure between the two species, which may be related to the discrepancy in fluorescence signals observed. We demonstrate that X. borealis oocytes are a viable heterologous system for expression of neuronal ion channels with some potential advantages over X. laevis oocytes for certain applications.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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