Conservation analysis of sequences flanking the testis-determining gene Sry in 17 mammalian species Gametogenesis and fertilization

Larney, Christian, Bailey, Timothy L. and Koopman, Peter (2015) Conservation analysis of sequences flanking the testis-determining gene Sry in 17 mammalian species Gametogenesis and fertilization. BMC Developmental Biology, 15 1: 34.1-34.14. doi:10.1186/s12861-015-0085-6


Author Larney, Christian
Bailey, Timothy L.
Koopman, Peter
Title Conservation analysis of sequences flanking the testis-determining gene Sry in 17 mammalian species Gametogenesis and fertilization
Journal name BMC Developmental Biology   Check publisher's open access policy
ISSN 1471-213X
Publication date 2015-10-06
Sub-type Article (original research)
DOI 10.1186/s12861-015-0085-6
Open Access Status DOI
Volume 15
Issue 1
Start page 34.1
End page 34.14
Total pages 14
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2016
Language eng
Formatted abstract
Background: Sex determination in mammals requires expression of the Y-linked gene Sry in the bipotential genital ridges of the XY embryo. Even minor delay of the onset of Sry expression can result in XY sex reversal, highlighting the need for accurate gene regulation during sex determination. However, the location of critical regulatory elements remains unknown. Here, we analysed Sry flanking sequences across many species, using newly available genome sequences and computational tools, to better understand Sry’s genomic context and to identify conserved regions predictive of functional roles.

Methods: Flanking sequences from 17 species were analysed using both global and local sequence alignment methods. Multiple motif searches were employed to characterise common motifs in otherwise unconserved sequence.

Results: We identified position-specific conservation of binding motifs for multiple transcription factor families, including GATA binding factors and Oct/Sox dimers. In contrast with the landscape of extremely low sequence conservation around the Sry coding region, our analysis highlighted a strongly conserved interval of ~106 bp within the Sry promoter (which we term the Sry Proximal Conserved Interval, SPCI). We further report that inverted repeats flanking murine Sry are much larger than previously recognised.

Conclusions: The unusually fast pace of sequence drift on the Y chromosome sharpens the likely functional significance of both the SPCI and the identified binding motifs, providing a basis for future studies of the role(s) of these elements in Sry regulation.
Keyword Gene regulation
Gonad
Sex determination
SRY
Testis
Y chromosome
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Institute for Molecular Bioscience - Publications
 
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