Comparison of concentration methods for rapid detection of hookworm ova in wastewater matrices using quantitative PCR

Gyawali, P., Ahmed, W., Jagals, P., Sidhu, J. P. S. and Toze, S. (2015) Comparison of concentration methods for rapid detection of hookworm ova in wastewater matrices using quantitative PCR. Experimental Parasitology, 159 160-167. doi:10.1016/j.exppara.2015.09.002

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Author Gyawali, P.
Ahmed, W.
Jagals, P.
Sidhu, J. P. S.
Toze, S.
Title Comparison of concentration methods for rapid detection of hookworm ova in wastewater matrices using quantitative PCR
Journal name Experimental Parasitology   Check publisher's open access policy
ISSN 1090-2449
Publication date 2015-12-01
Sub-type Article (original research)
DOI 10.1016/j.exppara.2015.09.002
Open Access Status File (Author Post-print)
Volume 159
Start page 160
End page 167
Total pages 8
Place of publication Maryland Heights, MO, United States
Publisher Academic Press
Collection year 2016
Language eng
Formatted abstract
Hookworm infection contributes around 700 million infections worldwide especially in developing nations due to poor sanitation. The effective recovery of hookworm ova from wastewater matrices is difficult due to their low concentrations and heterogeneous distribution. In this study, we compared the recovery rates of (i) four rapid hookworm ova concentration methods from municipal wastewater, and (ii) two concentration methods from sludge samples. Ancylostoma caninum ova were used as surrogate for human hookworm (Ancylostoma duodenale and Necator americanus). Known concentration of A. caninum hookworm ova were seeded into wastewater (treated and raw) and sludge samples collected from two wastewater treatment plants (WWTPs) in Brisbane and Perth, Australia. The A. caninum ova were concentrated from treated and raw wastewater samples using centrifugation (Method A), hollow fiber ultrafiltration (HFUF) (Method B), filtration (Method C) and flotation (Method D) methods. For sludge samples, flotation (Method E) and direct DNA extraction (Method F) methods were used. Among the four methods tested, filtration (Method C) method was able to recover higher concentrations of A. caninum ova consistently from treated wastewater (39-50%) and raw wastewater (7.1-12%) samples collected from both WWTPs. The remaining methods (Methods A, B and D) yielded variable recovery rates ranging from 0.2 to 40% for treated and raw wastewater samples. The recovery rates for sludge samples were poor (0.02-4.7), although, Method F (direct DNA extraction) provided 1-2 orders of magnitude higher recovery rate than Method E (flotation). Based on our results it can be concluded that the recovery rates of hookworm ova from wastewater matrices, especially sludge samples, can be poor and highly variable. Therefore, choice of concentration method is vital for the sensitive detection of hookworm ova in wastewater matrices.
Keyword Concentration methods
Hookworm ova
Public health
Recovery rate
Wastewater and sludge
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Public Health Publications
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Citation counts: TR Web of Science Citation Count  Cited 4 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 5 times in Scopus Article | Citations
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