Neuroprotective Effect of Resveratrol Against Methamphetamine-Induced Dopaminergic Apoptotic Cell Death in a Cell Culture Model of Neurotoxicity

Kanthasamy, Kavin, Gordon, Richard, Jin, Huajun, Anantharam, Vellareddy, Ali, Syed, Kanthasamy, Anumantha G. and Kanthasamy, Arthi (2011) Neuroprotective Effect of Resveratrol Against Methamphetamine-Induced Dopaminergic Apoptotic Cell Death in a Cell Culture Model of Neurotoxicity. Current Neuropharmacology, 9 1: 49-53. doi:10.2174/157015911795017353


Author Kanthasamy, Kavin
Gordon, Richard
Jin, Huajun
Anantharam, Vellareddy
Ali, Syed
Kanthasamy, Anumantha G.
Kanthasamy, Arthi
Title Neuroprotective Effect of Resveratrol Against Methamphetamine-Induced Dopaminergic Apoptotic Cell Death in a Cell Culture Model of Neurotoxicity
Journal name Current Neuropharmacology   Check publisher's open access policy
ISSN 1570-159X
1875-6190
Publication date 2011-03
Year available 2011
Sub-type Article (original research)
DOI 10.2174/157015911795017353
Volume 9
Issue 1
Start page 49
End page 53
Total pages 5
Place of publication Bussum, The Netherlands
Publisher Bentham Science Publishers
Language eng
Formatted abstract
A growing body of evidence suggests that oxidative stress-mediated cell death signaling mechanisms may exert neurotoxic effects of methamphetamine (MA)-induced dopaminergic neuronal loss. However, the means by which oxidative stress induced by MA causes neurodegeneration remains unclear. In recent years, resveratrol has garnered considerable attention owing to its antioxidant, anti-inflammatory, anti-aging, and neuroprotective properties. In the present study, we sought to investigate the neuroprotective effects of resveratrol against apoptotic cell death in a mesencephalic dopaminergic neuronal cell culture model of MA neurotoxicity. MA treatment in the N27 dopaminergic neuronal cell model produced a time-dependent activation of the apoptotic cascade involving caspase-3 and DNA fragmentation. We found that the caspase-3 activation preceded DNA fragmentation. Notably, treatment with resveratrol almost completely attenuated MA-induced caspase-3 activity, but only partially reduced apoptotic cell death. We conclude that the neuroprotective effect of resveratrol is at least in part mediated by suppression of caspase-3 dependent cell death pathways. Collectively, our results demonstrate that resveratrol can attenuate MA-induced apoptotic cell death and suggest that resveratrol or its analogs may have therapeutic benefits in mitigating MA-induced dopaminergic neurodegeneration.
Keyword Resveratrol
Drug abuse
Neuroprotection
Neurotoxicity
Oxidative Stress
Apoptosis
Oxidative Stress
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Tue, 13 Oct 2015, 17:44:05 EST by Richard Gordon on behalf of School of Medicine