ED27 trophoblast-like cells isolated from first-trimester chorionic villi are genetically identical to HeLa cells yet exhibit a distinct phenotype

Kniss, D. A., Xie, Y., Lia, Y., Kumar, S., Linton, E. A., Cohen, P., Fan-Havard, P., Redman, C. W. G. and Sargent, I. L. (2002) ED27 trophoblast-like cells isolated from first-trimester chorionic villi are genetically identical to HeLa cells yet exhibit a distinct phenotype. Placenta, 23 1: 32-43. doi:10.1053/plac.2001.0749


Author Kniss, D. A.
Xie, Y.
Lia, Y.
Kumar, S.
Linton, E. A.
Cohen, P.
Fan-Havard, P.
Redman, C. W. G.
Sargent, I. L.
Title ED27 trophoblast-like cells isolated from first-trimester chorionic villi are genetically identical to HeLa cells yet exhibit a distinct phenotype
Formatted title
ED27 trophoblast-like cells isolated from first-trimester chorionic villi are genetically identical to HeLa cells yet exhibit a distinct phenotype
Journal name Placenta   Check publisher's open access policy
ISSN 0143-4004
1532-3102
Publication date 2002-01
Sub-type Article (original research)
DOI 10.1053/plac.2001.0749
Open Access Status Not yet assessed
Volume 23
Issue 1
Start page 32
End page 43
Total pages 12
Place of publication London, United Kingdom
Publisher Elsevier
Language eng
Formatted abstract
ED27 trophoblast-like cells were prepared from human chorionic villus samples obtained at 9 weeks gestation and have been grown continuously in vitro without phenotypic drift for nearly a decade. These cells express many trophoblast markers, including cytokeratin, placental alkaline phosphatase (FLAP), secretion of 17β-estradiol, and a microvillous apical surface. The ED27 cell line is a useful model system for studies of placental cell biology and has been distributed to laboratories world-wide. However, experiments to investigate their relationship to primary villous cytotrophoblast have shown that these cells do not secrete detectable amounts of human chorionic gonadotropin in culture and, when digested with trypsin, disperse into individual cells. Furthermore, immunocytochemical studies demonstrated that, unlike villous cytotrophoblasts, ED27 cells were immunoreactive with monoclonal antibodies recognizing some HLA Class I antigens. This was not HLA-G, however, as would be expected if these cells originated from extravillous cytotrophoblasts, but rather classical HLA-A, B which is thought not to be expressed by any trophoblast subpopulations. These inconsistencies prompted us to question the authenticity of the continuous cell line as it now exists. Genetic haplotype analysis using the polymerase chain reaction (PCR) revealed that ED27 was genetically identically to the HeLa cell line. Inasmuch as HeLa cells have never been grown in the laboratory (DAK), the only possible origin of HeLa cell contamination of ED27 cells was the WISH cell line, and further PCR analysis revealed that this cell line was also genetically identical to HeLa. Like ED27 cells, HeLa cells and WISH cells synthesized small amounts of estrogen and were found to express PLAP and antigens recognized by the monoclonal antibodies ED822, directed against the syncytiotrophoblast, and J1B5 directed against villous cytotrophoblast. These results point out the need for adherence to rigorous and consistent quality control measures to assure the authenticity of cell lines used as in vitro model systems.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 30 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 31 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 09 Oct 2015, 20:16:39 EST by System User on behalf of Learning and Research Services (UQ Library)