A Cholesterol-Dependent Endocytic Mechanism Generates Midbody Tubules During Cytokinesis

Kettle, Emma, Page, Scott L., Morgan, Garry P., Malladi, Chandra S., Wong, Chin L., Boadle, Ross A., Marsh, Brad J., Robinson, Philip J. and Chircop, Megan (2015) A Cholesterol-Dependent Endocytic Mechanism Generates Midbody Tubules During Cytokinesis. Traffic, 16 11: 1174-1192. doi:10.1111/tra.12328


Author Kettle, Emma
Page, Scott L.
Morgan, Garry P.
Malladi, Chandra S.
Wong, Chin L.
Boadle, Ross A.
Marsh, Brad J.
Robinson, Philip J.
Chircop, Megan
Title A Cholesterol-Dependent Endocytic Mechanism Generates Midbody Tubules During Cytokinesis
Journal name Traffic   Check publisher's open access policy
ISSN 1600-0854
1398-9219
Publication date 2015-09-23
Year available 2015
Sub-type Article (original research)
DOI 10.1111/tra.12328
Open Access Status Not Open Access
Volume 16
Issue 11
Start page 1174
End page 1192
Total pages 19
Place of publication Malden, Massachusetts, United States
Publisher Wiley-Blackwell Publishing
Collection year 2016
Language eng
Abstract Cytokinesis is the final stage of cell division and produces two independent daughter cells. Vesicles derived from internal membrane stores, such as the Golgi, lysosomes, and early and recycling endosomes accumulate at the intracellular bridge (ICB) during cytokinesis. Here, we use electron tomography to show that many ICB vesicles are not independent but connected, forming a newly described ICB vesicular structure – narrow tubules that are often branched. These ‘midbody tubules’ labelled with horseradish peroxidase (HRP) within 10 min after addition to the surrounding medium demonstrating that they are derived from endocytosis. HRP-labelled vesicles and tubules were observed at the rim of the ICB after only 1 min, suggesting that midbody tubules are likely to be generated by local endocytosis occurring at the ICB rim. Indeed, at least one tubule was open to the extracellular space, indicative of a local origin within the ICB. Inhibition of cholesterol-dependent endocytosis by exposure to methyl-β-cyclodextrin and filipin reduced formation of HRP-labelled midbody tubules, and induced multinucleation following ICB formation. In contrast, dynamin inhibitors, which block clathrin-mediated endocytosis, induced multinucleation but had no effect on the formation of HRP-labelled midbody tubules. Therefore, our data reveal the existence of a cholesterol-dependent endocytic pathway occurring locally at the ICB, which contributes to the accumulation of vesicles and tubules that contribute to the completion of cytokinesis.
Keyword Abscission
Cytokinesis
Endocytosis
Midbody tubules
Vesicles
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Institute for Molecular Bioscience - Publications
 
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