In vivo characterization of microglial engulfment of dying neurons in the zebrafish spinal cord

Morsch, Marco, Radford, Rowan, Lee, Albert, Don, Emily K., Bedrock, Andrew P., Hall, Thomas E., Cole, Nicholas J. and Chung, Roger (2015) In vivo characterization of microglial engulfment of dying neurons in the zebrafish spinal cord. Frontiers in Cellular Neuroscience, 9 August: 321-321. doi:10.3389/fncel.2015.00321

Author Morsch, Marco
Radford, Rowan
Lee, Albert
Don, Emily K.
Bedrock, Andrew P.
Hall, Thomas E.
Cole, Nicholas J.
Chung, Roger
Title In vivo characterization of microglial engulfment of dying neurons in the zebrafish spinal cord
Journal name Frontiers in Cellular Neuroscience   Check publisher's open access policy
ISSN 1662-5102
Publication date 2015-08
Year available 2015
Sub-type Article (original research)
DOI 10.3389/fncel.2015.00321
Open Access Status DOI
Volume 9
Issue August
Start page 321
End page 321
Total pages 11
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Collection year 2016
Language eng
Formatted abstract
Microglia are specialized phagocytes in the vertebrate central nervous system (CNS). As the resident immune cells of the CNS they play an important role in the removal of dying neurons during both development and in several neuronal pathologies. Microglia have been shown to prevent the diffusion of damaging degradation products of dying neurons by engulfment and ingestion. Here we describe a live imaging approach that uses UV laser ablation to selectively stress and kill spinal neurons and visualize the clearance of neuronal remnants by microglia in the zebrafish spinal cord. In vivo imaging confirmed the motile nature of microglia within the uninjured spinal cord. However, selective neuronal ablation triggered rapid activation of microglia, leading to phagocytic uptake of neuronal debris by microglia within 20-30 min. This process of microglial engulfment is highly dynamic, involving the extension of processes toward the lesion site and consequently the ingestion of the dying neuron. 3D rendering analysis of time-lapse recordings revealed the formation of phagosome-like structures in the activated microglia located at the site of neuronal ablation. This real-time representation of microglial phagocytosis in the living zebrafish spinal cord provides novel opportunities to study the mechanisms of microglia-mediated neuronal clearance.
Keyword Zebrafish
Apoptotic Cells
Phagosome Maturation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Institute for Molecular Bioscience - Publications
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