Characterizing Enterovirus 71 and Coxsackievirus A16 Virus-like Particles Production in Insect Cells

Somasundaram, Balaji, Chang, Cindy, Fan, Yuan Y., Lim, Pei-Yin, Cardosa, Jane and Lua, Linda (2015) Characterizing Enterovirus 71 and Coxsackievirus A16 Virus-like Particles Production in Insect Cells. Methods, 95 38-45. doi:10.1016/j.ymeth.2015.09.023

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Author Somasundaram, Balaji
Chang, Cindy
Fan, Yuan Y.
Lim, Pei-Yin
Cardosa, Jane
Lua, Linda
Title Characterizing Enterovirus 71 and Coxsackievirus A16 Virus-like Particles Production in Insect Cells
Journal name Methods   Check publisher's open access policy
ISSN 1046-2023
Publication date 2015
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.ymeth.2015.09.023
Open Access Status File (Author Post-print)
Volume 95
Start page 38
End page 45
Total pages 30
Place of publication Maryland Heights, MO United States
Publisher Academic Press
Collection year 2016
Language eng
Formatted abstract
Enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) are two viruses commonly responsible for hand, foot and mouth disease (HFMD) in children. The lack of prophylactic or therapeutic measures against HFMD is a major public health concern. Insect cell-based EV71 and CVA16 virus-like particles (VLPs) are promising vaccine candidates against HFMD and are currently under development. In this paper, the influence of insect cell line, incubation temperature, and serial passaging effect of and stability of budded virus (BV) stocks on EV71 and CVA16 VLP production was investigated. Enhanced EV71 and CVA16 VLP production was observed in Sf9 cells compared to High FiveTM cells. Lowering the incubation temperature from the standard 27°C to 21°C increased the production of both VLPs in Sf9 cells. Serial passaging of CVA16 BV stocks in cell culture had a detrimental effect on the productivity of the structural proteins and the effect was observed with only 5 passages of BV stocks. A 2.7x higher production yield was achieved with EV71 compared to CVA16. High-resolution asymmetric flow field-flow fractionation couple with multi-angle light scattering (AF4-MALS) was used for the first time to characterize EV71 and CVA16 VLPs, displaying an average root mean square radius of 15 ± 1 nm and 15.3 ± 5.8 nm respectively. This study highlights the need for different approaches in the design of production process to develop a bivalent EV71 and CVA16 vaccine.
Keyword Enterovirus
Virus like particle
Insect cell
Field-flow fractionation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 29 Sep 2015, 11:14:18 EST by Linda Lua on behalf of Aust Institute for Bioengineering & Nanotechnology