Applying SWATH mass spectrometry to investigate human cervicovaginal fluid during the menstrual cycle

Vaswani, Kanchan, Ashman, Keith, Reed, Sarah, Salomon, Carlos, Sarker, Suchismita, Arraztoa, Jose A., Perez-Sepulveda, Alejandra, Illanes, Sebastian E., Kvaskoff, David, Mitchell, Murray D. and Rice, Gregory E. (2015) Applying SWATH mass spectrometry to investigate human cervicovaginal fluid during the menstrual cycle. Biology of Reproduction, 93 2: 1-10. doi:10.1095/biolreprod.115.128231

Author Vaswani, Kanchan
Ashman, Keith
Reed, Sarah
Salomon, Carlos
Sarker, Suchismita
Arraztoa, Jose A.
Perez-Sepulveda, Alejandra
Illanes, Sebastian E.
Kvaskoff, David
Mitchell, Murray D.
Rice, Gregory E.
Title Applying SWATH mass spectrometry to investigate human cervicovaginal fluid during the menstrual cycle
Journal name Biology of Reproduction   Check publisher's open access policy
ISSN 1529-7268
Publication date 2015-08-01
Sub-type Article (original research)
DOI 10.1095/biolreprod.115.128231
Open Access Status Not Open Access
Volume 93
Issue 2
Start page 1
End page 10
Total pages 10
Place of publication Madison, WI, United States
Publisher Society for the Study of Reproduction
Collection year 2016
Language eng
Formatted abstract
Inherent interindividual and intraindividual variation in the length of the menstrual cycle limits the accuracy of predicting days of peak fertility. To improve detection of days of peak fertility, a more detailed understanding of longitudinal changes in cervicovaginal fluid (CVF) biomarkers during the normal menstrual cycle is needed. The aim of this study, therefore, was to characterize longitudinal changes in CVF proteins during the menstrual cycle using a quantitative, data-independent acquisition mass spectrometry approach. Six serial samples were collected from women (n = 10) during the menstrual cycle. Samples were obtained at two time points for each phase of the cycle: early and late preovulatory, ovulatory, and postovulatory. Information-dependent acquisition (IDA) of mass spectra from all individual CVF samples was initially performed and identified 278 total proteins. Samples were then pooled by time of collection (n = 6 pools) and analyzed using IDA and information-independent acquisition (Sequential Windowed Acquisition of All Theoretical Mass Spectra [SWATH]). The IDA library generated contained 176 statistically significant protein identifications (P < 0.000158). The variation in the relative abundance of CVF proteins across the menstrual cycle was established by comparison with the SWATH profile against the IDA library. Using time-series, pooled samples obtained from 10 women, quantitative data were obtained by SWATH analysis for 43 CVF proteins. Of these proteins, 28 displayed significant variation in relative abundance during the menstrual cycle (assessed by ANOVA). Statistical significant changes in the relative expression of CVF proteins during preovulatory, ovulatory, and postovulatory phases of menstrual cycle were identified. The data obtained may be of utility not only in elucidating underlying physiological mechanisms but also as clinically useful biomarkers of fertility status.
Keyword Cervix
Female reproductive tract
Menstrual cycle
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2016 Collection
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