Gel chromatographic evaluation of the binding constant for the interaction of thiamin diphosphate with magnesium ion

Booth, CK, Nixon, PF and Winzor, DJ (1992) Gel chromatographic evaluation of the binding constant for the interaction of thiamin diphosphate with magnesium ion. Journal of Chromatography A, 609 1-2: 83-87. doi:10.1016/0021-9673(92)80151-J


Author Booth, CK
Nixon, PF
Winzor, DJ
Title Gel chromatographic evaluation of the binding constant for the interaction of thiamin diphosphate with magnesium ion
Journal name Journal of Chromatography A   Check publisher's open access policy
ISSN 0021-9673
Publication date 1992-09-18
Sub-type Article (original research)
DOI 10.1016/0021-9673(92)80151-J
Volume 609
Issue 1-2
Start page 83
End page 87
Total pages 5
Language eng
Subject 1602 Criminology
1308 Clinical Biochemistry
1313 Molecular Medicine
Abstract A simple gel chromatographic procedure is devised for characterizing the interactions of nucleotides and other coenzymes with metal ions. Its application is illustrated by determining the binding constant for the interaction of thiamin diphosphate with Mg2+ ion by frontal gel chromatography on Sephadex G-10. An association constant of 3200 (±400) M-1 is obtained for the interaction in 0.1 M Tris-HCl buffer (pH 7.6) supplemented with poly(ethylene glycol) (50 mg/ml) and mercaptoethanol (25 mM).
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
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