Immunohistochemical characterization of cloned lamb nephropathy

Dawson, Andrew J., King, Tim J., Wilmut, Ian, Harkness, Linda M., Kelly, Brian G. and Rhind, Susan M. (2004) Immunohistochemical characterization of cloned lamb nephropathy. Journal of Histochemistry and Cytochemistry, 52 12: 1657-1664. doi:10.1369/jhc.4A6367.2004

Author Dawson, Andrew J.
King, Tim J.
Wilmut, Ian
Harkness, Linda M.
Kelly, Brian G.
Rhind, Susan M.
Title Immunohistochemical characterization of cloned lamb nephropathy
Journal name Journal of Histochemistry and Cytochemistry   Check publisher's open access policy
ISSN 0022-1554
Publication date 2004-12
Sub-type Article (original research)
DOI 10.1369/jhc.4A6367.2004
Open Access Status Not yet assessed
Volume 52
Issue 12
Start page 1657
End page 1664
Total pages 8
Place of publication Thousand Oaks, CA, United States
Publisher Sage Publications
Language eng
Abstract Kidneys from lambs derived by nuclear transfer are frequently abnormal and are characterized by an enlarged pelvis and narrow medulla, consistent with lower urinary tract obstruction and development of variable hydronephrosis. The precise pathogenesis of this entity is unknown. Immunohistochemical staining for intermediate filaments was used to further characterize the lesions seen in this condition and was compared with age-matched control tissue. Major findings were upregulation of cytokeratin on damaged tubules, desmin and vimentin in undifferentiated mesenchyme, and smooth muscle actin in mesenchyme and on smooth muscle "collars" around dilated tubules. In addition, some cases showed reexpression of vimentin and desmin on proximal tubular epithelial cells. Taken together, these findings provide a valuable database for tracking the expression of intermediate filaments throughout renal development in sheep and have further characterized the nature of the response to injury by the developing kidney, a response that is characterized by proliferation of mesenchyme and both reexpression and upregulation of intermediate filaments within renal cells. In addition, the study has confirmed that the changes in cloned lamb nephropathy are established by day 85 of development.
Keyword Clones
Intermediate filaments
Nuclear transfer
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Created: Wed, 23 Sep 2015, 15:56:38 EST by Linda Harkness on behalf of Learning and Research Services (UQ Library)