Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark

Harkness, Linda, Rasmussen, Iben Anne, Erb, Karin and Kassem, Moustapha (2010) Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark. In Vitro Cellular and Developmental Biology - Animal, 46 3-4: 259-268. doi:10.1007/s11626-010-9281-7


Author Harkness, Linda
Rasmussen, Iben Anne
Erb, Karin
Kassem, Moustapha
Title Derivation and characterisation of hESC lines from supernumerary embryos, experience from Odense, Denmark
Journal name In Vitro Cellular and Developmental Biology - Animal   Check publisher's open access policy
ISSN 1071-2690
1557-8534
Publication date 2010-04
Sub-type Article (original research)
DOI 10.1007/s11626-010-9281-7
Open Access Status Not Open Access
Volume 46
Issue 3-4
Start page 259
End page 268
Total pages 10
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Abstract The derivation and characterisation of human embryonic stem cells provides a source of pluripotent stem cells with potential for clinical applications. Utilising locally sourced embryos from two IVF clinics, we derived and characterised five new cell lines for use in a non-clinical setting. Analysis of clinical data showed that the majority of embryos (94.5%) failed to reach the blastocyst stage of development and of all embryos, regardless of developmental status, 248 embryos were needed to create one stem cell line. From the number of embryos (69) which developed to the blastocyst stage 8.7% developed into cell lines. Using outgrowth of the whole blastocyst, we derived five new, unreported cell lines in Odense, Denmark between 2005 and 2006. Characterisation was carried out using RT-PCR, staining, karyotyping, EB formation and teratoma formation. The KMEB hESC lines will, in the future, be made available through the UK Stem Cell Bank ( http://www.ukstemcellbank.org.uk/ ).
Keyword Characterisation
Derivation
Human embryonic stem cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Wed, 23 Sep 2015, 15:37:10 EST by Linda Harkness on behalf of Scholarly Communication and Digitisation Service