Characterization of peroxisome proliferator-activated receptor alpha in normal rat mammary gland and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced mammary gland tumors from rats fed high and low fat diets

Roberts-Thomson, S. J. and Snyderwine, E. G. (2000) Characterization of peroxisome proliferator-activated receptor alpha in normal rat mammary gland and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced mammary gland tumors from rats fed high and low fat diets. Toxicology Letters, 118 1-2: 79-86.


Author Roberts-Thomson, S. J.
Snyderwine, E. G.
Title Characterization of peroxisome proliferator-activated receptor alpha in normal rat mammary gland and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced mammary gland tumors from rats fed high and low fat diets
Journal name Toxicology Letters   Check publisher's open access policy
ISSN 0378-4274
Publication date 2000
Sub-type Article (original research)
DOI 10.1016/S0378-4274(00)00265-4
Volume 118
Issue 1-2
Start page 79
End page 86
Total pages 8
Editor J.P. Kehrer
W. Dekant
Place of publication Ireland
Publisher Elsevier Science
Collection year 2000
Language eng
Subject C1
270199 Biochemistry and Cell Biology not elsewhere classified
730108 Cancer and related disorders
Abstract Normal Sprague-Dau ley rat mammary gland epithelial cells and mammary gland carcinomas induced by 2-amino-1 -methyl-6-phenylimidazo[4,5-b]pyridine, a carcinogen found in the diet, were examined for the expression of peroxisome proliferator-activated receptor alpha (PPAR alpha). PPAR alpha mRNA and protein was detected in normal and tumor tissue by reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry. By quantitative RT-PCR, carcinomas had a 12-fold higher expression than control mammary glands, a statistically significant difference. PPAR alpha expression was examined in carcinomas and normal tissues from rats on high fat (23.5/% corn oil) and low fat (5% corn oil) diets. Although neither carcinomas, nor control tissues showed statistically significant differences between the two diet groups, PPAR alpha expression was the highest in carcinomas from rats on the high fat diet. The expression of PPAR alpha in normal mammary gland and its significant elevation in mammary gland carcinomas raises the possibility of its involvement in mammary gland physiology and pathophysiology. (C) 2000 Published by Elsevier Science Ireland Ltd. All rights reserved.
Keyword Toxicology
Ppar Alpha
Dietary Fat
Phip
Rat
Mammary Gland
Tumor
Sprague-dawley Rats
Human Breast-cancer
Polymerase Chain-reaction
Ppar-alpha
Metabolic-activation
Adduct Formation
Acids Activate
Retinoic Acid
Messenger-rna
In-vitro
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Pharmacy Publications
 
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