Lepr(db) mouse model of type 2 diabetes: pancreatic islet isolation and live-cell 2-photon imaging of intact islets.

Do, Oanh H, Low, Jiun T and Thorn, Peter (2015) Lepr(db) mouse model of type 2 diabetes: pancreatic islet isolation and live-cell 2-photon imaging of intact islets.. Journal of Visualized Experiments, 99 99: e52632-e52632. doi:10.3791/52632


Author Do, Oanh H
Low, Jiun T
Thorn, Peter
Title Lepr(db) mouse model of type 2 diabetes: pancreatic islet isolation and live-cell 2-photon imaging of intact islets.
Journal name Journal of Visualized Experiments   Check publisher's open access policy
ISSN 1940-087X
Publication date 2015-05-11
Year available 2015
Sub-type Article (original research)
DOI 10.3791/52632
Volume 99
Issue 99
Start page e52632
End page e52632
Total pages 8
Place of publication Somerville, MA United States
Publisher Journal of Visualized Experiments
Collection year 2016
Language eng
Formatted abstract
Type 2 diabetes is a chronic disease affecting 382 million people in 2013, and is expected to rise to 592 million by 2035 (1). During the past 2 decades, the role of beta-cell dysfunction in type 2 diabetes has been clearly established (2). Research progress has required methods for the isolation of pancreatic islets. The protocol of the islet isolation presented here shares many common steps with protocols from other groups, with some modifications to improve the yield and quality of isolated islets from both the wild type and diabetic Lepr(db) (db/db) mice. A live-cell 2-photon imaging method is then presented that can be used to investigate the control of insulin secretion within islets.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biomedical Sciences Publications
 
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Created: Thu, 17 Sep 2015, 08:10:19 EST by Assoc Prof Peter Thorn on behalf of School of Biomedical Sciences