The Effect of Glucose on the Release and Bioactivity of Exosome from First Trimester Totrophoblast Cells

Rice, Gregory E., Scholz-Romero, Katherin, Sweeney, Emma, Peiris, Hassendrini, Kobayashi, Miharu, Duncombe, Gregory, Mitchell, Murray D. and Salomon, Carlos (2015) The Effect of Glucose on the Release and Bioactivity of Exosome from First Trimester Totrophoblast Cells. Journal of Clinical Endocrinology and Metabolism, 100 10: E1280-E1288. doi:10.1210/jc.2015-2270


Author Rice, Gregory E.
Scholz-Romero, Katherin
Sweeney, Emma
Peiris, Hassendrini
Kobayashi, Miharu
Duncombe, Gregory
Mitchell, Murray D.
Salomon, Carlos
Title The Effect of Glucose on the Release and Bioactivity of Exosome from First Trimester Totrophoblast Cells
Journal name Journal of Clinical Endocrinology and Metabolism   Check publisher's open access policy
ISSN 0021-972X
1945-7197
Publication date 2015-10-01
Sub-type Article (original research)
DOI 10.1210/jc.2015-2270
Open Access Status Not Open Access
Volume 100
Issue 10
Start page E1280
End page E1288
Total pages 9
Place of publication Chevy Chase, MD United States
Publisher The Endocrine Society
Collection year 2016
Language eng
Abstract Context: Hyperglycaemia and hypoxia are risk factors of metabolic complication during pregnancy. The interactions between oxygen and glucose sensing pathways that regulate exosome bioactivity from placental cells, however, have not been established. Objective: The aim of this study was to test the hypothesis that exosomal signalling by placental cells (defined as the number of exosomes released per unit time and their bioactivity) is responsive to extracellular glucose concentration. Methods: First trimester primary trophoblast cells were incubated with D-glucose (5 mM or 25 mM) under 1%, 3% or 8% O2 for 48 h. Exosomes were isolated from cell-conditioned media by differential and buoyant density centrifugation. The total number of exosome vesicles was determined by quantifying immunoreactive exosomal CD63 by the ELISA kit. The effect of exosomes on cytokine (GM-CSF, IL-2, IL-4, IL-6. IL-8, IL-10, IFN-γ and TNF-α) release from endothelial cells was established by the protein solution array analysis (Bioplex-200). Results: Glucose (25mM) significantly increased the release of exosomes from trophoblast cells at all oxygen tensions tested (by approximately 2-fold when compared to controls, p <0.001). Exosomes (100-μ g exosomal protein/ml) released from trophoblast cells significantly increased (p<0.05) the release of all cytokines from human umbilical vein endothelial cells (HUVEC) when compared to the control (i.e. cells without exosomes), with the exception of IL-2 and IL-10 (p>0.05). Conclusions: The effects of high glucose on exosomes bioactivity may be recapitulated and is of clinical relevance in vivo in association with maternal insulin resistance (resulting in hyperglycaemia) and preeclampsia (associated with placental insufficiency and hypoxia).
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
Official 2016 Collection
School of Medicine Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
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Created: Tue, 15 Sep 2015, 21:02:00 EST by Roheen Gill on behalf of UQ Centre for Clinical Research