Laser-based patterning for transfected cell microarrays

Hook, Andrew L., Creasey, Rhiannon, Hayes, Jason P., Thissen, Helmut and Voelcker, Nicolas H. (2009) Laser-based patterning for transfected cell microarrays. Biofabrication, 1 4: . doi:10.1088/1758-5082/1/4/045003


Author Hook, Andrew L.
Creasey, Rhiannon
Hayes, Jason P.
Thissen, Helmut
Voelcker, Nicolas H.
Title Laser-based patterning for transfected cell microarrays
Journal name Biofabrication   Check publisher's open access policy
ISSN 1758-5082
1758-5090
Publication date 2009
Year available 2009
Sub-type Article (original research)
DOI 10.1088/1758-5082/1/4/045003
Volume 1
Issue 4
Total pages 9
Place of publication Bristol, United Kingdom
Publisher Institute of Physics Publishing
Language eng
Abstract The spatial control over biomolecule- and cell-surface interactions is of great interest to a broad range of biomedical applications, including sensors, implantable devices and cell microarrays. Microarrays in particular require precise spatial control and the formation of patterns with microscale features. Here, we have developed an approach specifically designed for transfected cell microarray (TCM) applications that allows microscale spatial control over the location of both DNA and cells on highly doped p-type silicon substrates. This was achieved by surface modification, involving plasma polymerization of allylamine, grafting of poly(ethylene glycol) and subsequent excimer laser ablation. DNA could be delivered in a spatially defined manner using ink-jet printing. In addition, electroporation was investigated as an approach to transfect attached cells with adsorbed DNA and good transfection efficiencies of approximately 20% were observed. The ability of the microstructured surfaces to spatially direct both DNA adsorption and cell attachment was demonstrated in a functional TCM, making this system an exciting platform for chip-based functional genomics.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemical Engineering Publications
 
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Created: Tue, 15 Sep 2015, 10:33:08 EST by Noni Creasey on behalf of School of Chemical Engineering