Molecular characterization of begomovirus infecting yard long bean (Vigna unguiculata subsp. sesquipedalis L.) in java, Indonesia

Nurulita, Saru, Hidayat, Sri Hendrastuti, Mutaqin, Kikin Hamzah and Thomas, John Edward (2015) Molecular characterization of begomovirus infecting yard long bean (Vigna unguiculata subsp. sesquipedalis L.) in java, Indonesia. Biotropia, 22 1: 53-60. doi:10.11598/btb.2015.22.1.401

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ369281_OA.pdf Full text (open access) application/pdf 1.35MB 0

Author Nurulita, Saru
Hidayat, Sri Hendrastuti
Mutaqin, Kikin Hamzah
Thomas, John Edward
Title Molecular characterization of begomovirus infecting yard long bean (Vigna unguiculata subsp. sesquipedalis L.) in java, Indonesia
Formatted title
Molecular characterization of begomovirus infecting yard long bean (Vigna unguiculata subsp. sesquipedalis L.) in java, Indonesia
Journal name Biotropia   Check publisher's open access policy
ISSN 1907-770X
0215-6334
Publication date 2015
Sub-type Article (original research)
DOI 10.11598/btb.2015.22.1.401
Open Access Status File (Publisher version)
Volume 22
Issue 1
Start page 53
End page 60
Total pages 8
Place of publication Bogor, Indonesia
Publisher Seameo Biotrop - Southeast Asian Regional Centre for Tropical Biology
Collection year 2016
Language eng
Formatted abstract
Begomovirus was identified as one of the causal agents causing yellow mosaic disease on yard long bean (Vigna unguiculata subsp. sesquipedalis L.) in Java. Research was conducted to characterize the virus based on its nucleotide sequences. Research was started by field survey to collect leaf samples, followed by virus detection using polymerase chain reaction and analysis of virus sequences. Samples from Tegal, Klaten, Magelang, Cirebon, Subang, and Bogor were positively infected by Begomovirus based on specific viral DNA amplification. Sequence analysis indicated that Begomovirus infecting yard long bean belongs to the same group with Mungbean yellow mosaic India virus (MYMIV) from Bangladesh, India, Pakistan, and Nepal. Further analysis showed the conserved region of Begomovirus around Common Region, i.e. “TATA box” Sequence, Hair Pin Loop Structure, Repetitive Sequence, and the Conserved Nonanucleotide Sequence TAATATTAC.
Keyword Begomovirus
Common region
DNA sequencing
I-ELISA
MYMIV
PCR
Yard long bean
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes DOI is broken

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Alliance for Agriculture and Food Innovation
Official 2016 Collection
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 15 Sep 2015, 02:07:16 EST by System User on behalf of Scholarly Communication and Digitisation Service