Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources

De Smet, Birgit., Mayo, Mark., Peeters, Charlotte., Zlosnik, James E.A., Spilker, Theodore., Hird, Trevor J., Li Puma, John J., Kidd, Timothy J., Kaestli, Mirjam., Ginther, Jennifer L., Wagner, David M., Keim, Paul., Bell, Scott C., Jacobs, Jan A., Currie, Bart J. and Vandamme, Peter. (2015) Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources. International Journal of Systematic and Evolutionary Microbiology, 65 7: 2265-2271. doi:10.1099/ijs.0.000251


Author De Smet, Birgit.
Mayo, Mark.
Peeters, Charlotte.
Zlosnik, James E.A.
Spilker, Theodore.
Hird, Trevor J.
Li Puma, John J.
Kidd, Timothy J.
Kaestli, Mirjam.
Ginther, Jennifer L.
Wagner, David M.
Keim, Paul.
Bell, Scott C.
Jacobs, Jan A.
Currie, Bart J.
Vandamme, Peter.
Title Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources
Journal name International Journal of Systematic and Evolutionary Microbiology   Check publisher's open access policy
ISSN 1466-5026
1466-5034
Publication date 2015-07-01
Year available 2015
Sub-type Article (original research)
DOI 10.1099/ijs.0.000251
Open Access Status Not Open Access
Volume 65
Issue 7
Start page 2265
End page 2271
Total pages 7
Place of publication Reading, Berks, United Kingdom
Publisher Society for General Microbiology
Collection year 2016
Language eng
Formatted abstract
Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Bcc L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9 %, respectively) and towards established Bcc species (4.0 and 3.9 %, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156T ( = CCUG 65686T) and Burkholderia territorii sp. nov. with type strain LMG 28158T ( = CCUG 65687T) are proposed for Bcc B and Bcc L bacteria, respectively.
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
Child Health Research Centre Publications
 
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