Synchrotron-based X-ray absorption near-edge spectroscopy imaging for laterally resolved speciation of selenium in fresh roots and leaves of wheat and rice

Wang, Peng., Menzies, Neal W., Lombi, Enzo., McKenna, Brigid A., James, Simon., Tang, Caixian. and Kopittke, Peter M. (2015) Synchrotron-based X-ray absorption near-edge spectroscopy imaging for laterally resolved speciation of selenium in fresh roots and leaves of wheat and rice. Journal of Experimental Botany, 66 15: 4795-4806. doi:10.1093/jxb/erv254


Author Wang, Peng.
Menzies, Neal W.
Lombi, Enzo.
McKenna, Brigid A.
James, Simon.
Tang, Caixian.
Kopittke, Peter M.
Title Synchrotron-based X-ray absorption near-edge spectroscopy imaging for laterally resolved speciation of selenium in fresh roots and leaves of wheat and rice
Journal name Journal of Experimental Botany   Check publisher's open access policy
ISSN 1460-2431
0022-0957
Publication date 2015-05-26
Year available 2015
Sub-type Article (original research)
DOI 10.1093/jxb/erv254
Open Access Status Not Open Access
Volume 66
Issue 15
Start page 4795
End page 4806
Total pages 12
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2016
Language eng
Formatted abstract
Knowledge of the distribution of selenium (Se) species within plant tissues will assist in understanding the mechanisms of Se uptake and translocation, but in situ analysis of fresh and highly hydrated plant tissues is challenging. Using synchrotron-based fluorescence X-ray absorption near-edge spectroscopy (XANES) imaging to provide laterally resolved data, the speciation of Se in fresh roots and leaves of wheat (Triticum aestivum L.) and rice (Oryza sativa L.) supplied with 1 μM of either selenate or selenite was investigated. For plant roots exposed to selenate, the majority of the Se was efficiently converted to C-Se-C compounds (i.e. methylselenocysteine or selenomethionine) as selenate was transported radially through the root cylinder. Indeed, even in the rhizodermis which is exposed directly to the bulk solution, only 12–31% of the Se was present as uncomplexed selenate. The C-Se-C compounds were probably sequestered within the roots, whilst much of the remaining uncomplexed Se was translocated to the leaves—selenate accounting for 52–56% of the total Se in the leaves. In a similar manner, for plants exposed to selenite, the Se was efficiently converted to C-Se-C compounds within the roots, with only a small proportion of uncomplexed selenite observed within the outer root tissues. This resulted in a substantial decrease in translocation of Se from the roots to leaves of selenite-exposed plants. This study provides important information for understanding the mechanisms responsible for the uptake and subsequent transformation of Se in plants.
Keyword Fluorescence-XANES imaging
laterally resolved speciation
selenium uptake
speciation
transformation
translocation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Agriculture and Food Sciences
Official 2016 Collection
 
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