Effect of chronic alcohol ingestion on hepatic folate distribution in the rat

Hidiroglou, N, Camilo, ME, Beckenhauer, HC, Tuma, DJ, Barak, AJ, Nixon, PF and Selhub, J (1994) Effect of chronic alcohol ingestion on hepatic folate distribution in the rat. Biochemical Pharmacology, 47 9: 1561-1566. doi:10.1016/0006-2952(94)90532-0

Author Hidiroglou, N
Camilo, ME
Beckenhauer, HC
Tuma, DJ
Barak, AJ
Nixon, PF
Selhub, J
Title Effect of chronic alcohol ingestion on hepatic folate distribution in the rat
Journal name Biochemical Pharmacology   Check publisher's open access policy
ISSN 0006-2952
Publication date 1994-04-29
Sub-type Article (original research)
DOI 10.1016/0006-2952(94)90532-0
Volume 47
Issue 9
Start page 1561
End page 1566
Total pages 6
Language eng
Subject 3004 Pharmacology
Abstract The mechanism by which ethanol impairs folate metabolism remains uncertain. In the present study, we used our new technique (affinity/HPLC) for folate analysis to study the effect of chronic alcohol ingestion on the content and distribution of folates in livers. Twelve male Sprague-Dawley rats (180 g) were divided into two groups, and fed for 4 weeks with Lieber-DeCarli semi-liquid isocaloric diets, with and without 5% ethanol. Livers were extracted in boiling, pH 9.3 borate buffers containing ascorbate/dithioerythritol. Folates in the supernatant fractions were purified by affinity chromatography and analyzed using ion pair high performance liquid chromatography. The data obtained showed that hepatic folate distribution in alcohol-treated rats differed from that of control animals in two ways. Livers from the ethanol-fed rats, when compared with those from control rats, exhibited increases in the percent concentrations of methylated tetrahydrofolates (21.46 ± 2.21 vs 14.8 ± 1.23), decreases in the percent concentrations of formylated tetrahydrofolates (25.62 ± 4.02 vs 46.18 ± 2.65) and higher concentrations of unsubstituted tetrahydrofolates (52.91 ± 3.84 vs 38.88 ± 2.50). In addition, alcohol ingestion was associated with longer glutamate chains of the folate molecules, characterized by lower relative concentrations of pentaglutamyl folates (29 vs 48%), and higher relative concentrations of hexa- and heptaglutamyl folates (55 vs 46% and 15 vs 6%) when compared with controls. The data are discussed in relation to possibility that alcohol exerts its effect through: (1) inhibition of B12-dependent methyl transfer from methyltetrahydrofolate to homocysteine; (2) diversion of formylated tetrahydrofolates toward serine synthesis; and (3) interaction of acetaldehyde with tetrahydrofolates, thereby interfering with folate coenzyme metabolism.
Keyword acetaldehyde
folate analysis
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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